ZFIN ID: ZDB-FIG-200129-34
Fallata et al., 2019 - Intracellular Localization in Zebrafish Muscle and Conserved Sequence Features Suggest Roles for Gelatinase A Moonlighting in Sarcomere Maintenance. Biomedicines   7(4) Full text @ Biomedicines
Anatomical Term:
Stage: Protruding-mouth

Figure 1

Mmp2 is expressed ubiquitously from early development and accumulates in a striated pattern within the skeletal muscle. (A) Composite confocal projections of a 72 hpf embryo stained with anti-Mmp2 exhibiting labeling throughout the embryo with notable accumulation in the skeletal muscle (scale bar = 200 µm). (B) High magnification view of a single confocal section through the trunk musculature (indicated by the inset) showing strong labeling of the myotome boundary (upper left corner), and striated staining in myofibrils (scale bar = 10 µm). (C) RNASeq data showing absolute abundance of mmp2 transcripts in embryos from fertilization to five days post-fertilization (dpf). (D) Immunoblot of whole embryo homogenates (350 µg per lane) made from 2 hpf (cleavage), 5 hpf (50% epiboly), 12 hpf (early somitogenesis), 18 hpf (late somitogenesis), 24 hpf (prim-5), 48 hpf (long pec), and 72 hpf (protruding mouth) embryos probed with anti-Mmp2. Immunoreactivity is detected in embryos after 12 h of development, with bands at the expected mobility for full-length Mmp2 (72 kD) and stronger bands at 44 and 20 kD, which combine to give the expected size for activated Mmp2 (64 kD).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mmp2 Ab1-mmp2 WT standard conditions Protruding-mouth trunk musculature IHC
Antibody Labeling Details No data available
Phenotype Details No data available
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