ZFIN ID: ZDB-FIG-191230-1319
Mazzolini et al., 2019 - Gene expression profiling reveals a conserved microglia signature in larval zebrafish. Glia   68(2):298-315 Full text @ Glia
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Days 7-13

Fig. 1

Development of the microglia population in larval zebrafish. (a) Representative confocal images are shown to illustrate zebrafish larval head development from 1 to 7 days postfertilization (dpf) and microglial cell distribution throughout the developing brain. Upper panels correspond to a brightfield transmission image and lower panels represent the maximum intensity projection of 4C4+ microglia (magenta) at each developmental stage. Microglia start colonizing the brain (dotted line) at 3 dpf whereas signal can be detected in the retina from 1 dpf onwards. Scale bar represents 50 μm. (b) Upper panels show 4C4 antibody immunohistochemistry, lower panels show segmented images of microglia morphology at 3, 5, and 7 dpf using the Imaris surface tool. Microglia morphology changes from amoeboid (1 dpf) to ramified (7 dpf) with an intermediate feature at 5 dpf. Scale bar represents 10 μm. (c) Schematic representation of the protocol used to isolate 4C4+ microglia from larval zebrafish brains at 3, 5, and 7 dpf. All images represent maximum intensity projections of confocal stacks. Images were captured using a Zeiss LSM710 confocal microscope with a 20×/NA 0.8 objective [Color figure can be viewed at http://wileyonlinelibrary.com]

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-4C4 IHC WT control Prim-5 optic cup microglial cell
IHC Long-pec retina microglial cell
IHC Protruding-mouth brain microglial cell
IHC Protruding-mouth retina microglial cell
IHC Day 5 brain microglial cell
IHC Day 5 retina microglial cell
IHC Days 7-13 brain microglial cell
IHC Days 7-13 retina microglial cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Glia for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Glia