ZFIN ID: ZDB-FIG-191224-2
Villani et al., 2019 - Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment. Developmental Cell   49(1):77-88.e7 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 3 Cloning and Characterization of Slc37a2, the Gene Underlying the blb Phenotype

(A) Schematic of the slc37a2 locus. Sequences of the NY007/t30301 allele (C/T mutation) and NA150/t30913 allele (T/A mutation).

(B) Dorsal view of a 4 dpf Slc37a2 knockout generated with CRISPR-Cas9 as shown in (A). Scale bar, 30 μm.

(C) Dorsal view of a representative immunostaining of a 4 dpf embryo where microglia are in green Tg(mpeg:GFP-caax) and Slc37a2 in red. Scale bar, 30 μm.

(D) Magnification of (C). Scale bar, 15 μm. The yellow arrow points to a phagosome positive for Slc37a2.

(E) Western blot analysis of Slc37a2 (55 kDa) and Tubulin (48 kDa) in wild-type, blbNY007, and blbNI150.

(F) Immunostainings of Slc37a2 (cyan) and Lamp1 (red) in RAW264.7. Scale bar, 5 μm.

(G and H) Mass-spectrometry data showing normalized intensities of glucose 6-phosphate (G) not significant, and D-gluconate-6-phosphate (H) significant, p value < 0.0001, in blbNY007 and wild-type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP zh901Tg standard conditions Day 4 microglial cell IFL
slc37a2 Ab2-slc37a2 WT standard conditions Day 4 whole organism WB
Ab2-slc37a2 slc37a2t30301/t30301 standard conditions Day 4 Not Detected whole organism WB
Ab2-slc37a2 slc37a2t30913/t30913 standard conditions Day 4 Not Detected whole organism WB
Ab2-slc37a2 zh901Tg standard conditions Day 4 microglial cell phagocytic vesicle IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slc37a2t30301/t30301 standard conditions Day 4 head 6-phospho-D-gluconate increased amount, abnormal
Day 4 whole organism slc37a2 expression absent, abnormal
slc37a2t30913/t30913 standard conditions Day 4 whole organism slc37a2 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell