ZFIN ID: ZDB-FIG-191220-46
Debiais-Thibaud et al., 2019 - Skeletal mineralisation in association with type X collagen expression is an ancestral feature for jawed vertebrates. Molecular Biology and Evolution   36(10):2265-2276 Full text @ Mol Bio Evol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 6

Fig. 4.

Expression of zebrafish col10a1a and col10a1b and Xenopus Col10a1 during tooth development detected with in situ hybridization. (A, B) col10a1a expression in whole-mount zebrafish larvae at 4 and 6 days post fertilization (dpf). (C) col10a1a expression in post in situ paraffin sections of zebrafish larvae at 5 dpf. (D, E) col10a1b expression in whole-mount zebrafish larvae at 4 and 6 dpf. (F) col10a1b expression in post in situ paraffin sections of zebrafish larvae at 4 dpf. (G) HE staining of a longitudinal section through a developing tooth bud of a Xenopus larva at stage NF57. (H, I) Col10a1 expression in Xenopus tooth bud on successive transversal sections. Open arrowheads point at developing pharyngeal teeth and asterisk marks the mineralizing matrix. Scale bars: 100 µm (A, B, D, E), 50 µm (C, F), 20 µm (G, H, I). Abbreviations: amel., ameloblasts; cl, cleithrum; d, dentary; m, maxilla; od., odontoblasts; op, opercle; p, parasphenoid; sec. amel., secretory ameloblasts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col10a1a WT standard conditions Day 4 ceratobranchial 5 tooth ISH
Day 4 cleithrum ISH
Day 4 dentary ISH
Day 4 ethmoid cartilage ISH
Day 4 maxilla ISH
Day 4 opercle ISH
Day 5 ceratobranchial 5 tooth ISH
Day 5 cleithrum ISH
Day 5 dentary ISH
Day 5 ethmoid cartilage ISH
Day 5 maxilla ISH
Day 5 opercle ISH
Day 6 ceratobranchial 5 tooth ISH
Day 6 cleithrum ISH
Day 6 dentary ISH
Day 6 ethmoid cartilage ISH
Day 6 maxilla ISH
Day 6 opercle ISH
col10a1b WT standard conditions Day 4 ceratobranchial 5 tooth ISH
Day 4 dental papilla ISH
Day 6 ceratobranchial 5 tooth ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular Biology and Evolution for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol Bio Evol