ZFIN ID: ZDB-FIG-190723-1455
Coronado et al., 2019 - Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile. Frontiers in immunology   10:610 Full text @ Front Immunol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Figure 3

Lymphoid cells are present in the intestine as early as 5 dpf and are able to respond to food antigens. (A–D) Lateral view of the intestine from a Tg(lck:lck-eGFP) larva showing fluorescently labelled lymphoid cells (green). (E–H) Higher magnification of lymphocytes under the different conditions. (I) Quantification of lymphoid cells under the different conditions (n: naive; CTRL: control; INFL: inflammation) and time points (T0: 5 dpf; T4: 9 dpf). (J–O) T cells in control (J) or inflamed (M) intestine do not colocalize with EdU+ cells (K,L,N,O). (P) Example of migration of T cells (arrowhead) in a Tg(lck:lck-eGFP) larvae fed with control diet (derived from Supplementary Movie 1). (Q) Example of the migration of T cells (arrowheads) in a Tg(lck:lck-eGFP) larva fed with inflammatory diet (derived from Supplementary Movie 2). (R) Relative mRNA levels of ccl25 and ccr9a were analyzed in the intestine of control and inflamed larvae. Data was normalized against rpl13a and compared to naïve or maintenance condition (dotted line). *p < 0.05; **p < 0.01; ***p < 0.001; ****p > 0.0001. Scale bar for A–D 200 μm and J–O 100 μm. Experiments were done at least in three biological replicates with 20 individuals per condition.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccl25a WT diet Days 7-13 intestine RTPCR
EGFP cz2Tg control Days 7-13 intestine lymphoid progenitor cell IFL
cz2Tg diet Days 7-13 intestine lymphoid progenitor cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT diet Days 7-13 intestine ccl25a expression increased amount, abnormal
cz2Tg diet Days 7-13 intestine lymphoid progenitor cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol