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ZFIN ID: ZDB-FIG-190423-4
Li et al., 2018 - Endodermal pouch-expressed dmrt2b is important for pharyngeal cartilage formation.. Biology Open   7(12) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4 dmrt2b facilitates CNC cell proliferation and differentiation. (A,C,D) Representative confocal sections of pH3-positive cells in the first and second pharyngeal arches at the indicated stages. (B,E) Percentage of pH3-positive cells among GFP-positive CNC cells. n≥6 embryos for each condition. Significance was analyzed using unpaired t-tests. *P<0.05; **P<0.01; ***P<0.001. (F) Expression levels of col2a1 mRNA at 72 hpf. Red circles indicate the pharyngeal region. The left panels are lateral views with anterior to the left and the right panels are ventral views with the anterior to the top. (G,H) Immunostaining of Col2 protein in pharyngeal cartilages at 84 hpf. dmrt2b mutants (G) and Tg(fli1:EGFP) embryos injected with 4 ng dmrt2b MO (H) were stained with the indicated fluorescent antibodies. PA, pharyngeal arch; pq, palatoquadrate; ch, ceratohyal. Scale bars: 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a TU control Protruding-mouth pharyngeal arch ISH
dmrt2bioz21/ioz21(TU) standard conditions Protruding-mouth pharyngeal arch ISH
EGFP ba2Tg control Prim-5 pharyngeal arch 1 cell IHC
Prim-5 pharyngeal arch 2 cell IHC
Prim-25 pharyngeal arch 1 cell IHC
Long-pec pharyngeal arch 1 cell IHC
ba2Tg + MO3-dmrt2b standard conditions Prim-5 pharyngeal arch 1 cell IHC
Prim-5 pharyngeal arch 2 cell IHC
Prim-25 pharyngeal arch 1 cell IHC
Prim-25 pharyngeal arch 2 cell IHC
Long-pec pharyngeal arch 1 cell IHC
Long-pec pharyngeal arch 2 cell IHC
y1Tg control Protruding-mouth pharyngeal arch IHC
y1Tg + MO3-dmrt2b standard conditions Protruding-mouth pharyngeal arch IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC TU control Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth palatoquadrate cartilage
IHC dmrt2bioz21/ioz21(TU) standard conditions Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth palatoquadrate cartilage
IHC y1Tg control Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth palatoquadrate cartilage
IHC y1Tg + MO3-dmrt2b standard conditions Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth palatoquadrate cartilage
Ab36-h3 IHC ba2Tg control Prim-5 pharyngeal arch 1 cell
IHC Prim-5 pharyngeal arch 2 cell
IHC Prim-25 pharyngeal arch 1 cell
IHC Prim-25 pharyngeal arch 2 cell
IHC Long-pec pharyngeal arch 1 cell
IHC Long-pec pharyngeal arch 2 cell
IHC ba2Tg + MO3-dmrt2b standard conditions Prim-5 pharyngeal arch 1 cell
IHC Prim-5 pharyngeal arch 2 cell
IHC Prim-25 pharyngeal arch 1 cell
IHC Prim-25 pharyngeal arch 2 cell
IHC Long-pec pharyngeal arch 1 cell
IHC Long-pec pharyngeal arch 2 cell
Phenotype Details
Fish Conditions Stage Phenotype
ba2Tg + MO3-dmrt2b standard conditions Prim-5 pharyngeal arch 1 cell Ab36-h3 labeling decreased amount, abnormal
Prim-5 pharyngeal arch 1 cell EGFP expression decreased amount, abnormal
Prim-5 pharyngeal arch 2 cell Ab36-h3 labeling decreased amount, abnormal
Prim-5 pharyngeal arch 2 cell EGFP expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 cell EGFP expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 cell Ab36-h3 labeling decreased amount, abnormal
Prim-25 pharyngeal arch 2 cell EGFP expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 cell Ab36-h3 labeling decreased amount, abnormal
Long-pec pharyngeal arch 1 cell EGFP expression decreased amount, abnormal
Long-pec pharyngeal arch 1 cell Ab36-h3 labeling decreased amount, abnormal
Long-pec pharyngeal arch 2 cell Ab36-h3 labeling decreased amount, abnormal
Long-pec pharyngeal arch 2 cell EGFP expression decreased amount, abnormal
dmrt2bioz21/ioz21(TU) standard conditions Protruding-mouth ceratohyal cartilage ab1-col2a labeling decreased amount, abnormal
Protruding-mouth ceratohyal cartilage decreased amount, abnormal
Protruding-mouth palatoquadrate cartilage ab1-col2a labeling decreased amount, abnormal
Protruding-mouth palatoquadrate cartilage decreased amount, abnormal
Protruding-mouth pharyngeal arch col2a1a expression decreased amount, abnormal
y1Tg + MO3-dmrt2b standard conditions Protruding-mouth ceratohyal cartilage decreased amount, abnormal
Protruding-mouth ceratohyal cartilage ab1-col2a labeling decreased amount, abnormal
Protruding-mouth palatoquadrate cartilage decreased amount, abnormal
Protruding-mouth palatoquadrate cartilage ab1-col2a labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open