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ZFIN ID: ZDB-FIG-190404-3
Ton et al., 2018 - Collagen COL22A1 maintains vascular stability and mutations in COL22A1 are potentially associated with intracranial aneurysms. Disease models & mechanisms   11(12) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 2 col22a1 expression in the head and trunk region analyzed by FISH and FISH/immunofluorescence. (A-I) Expression of col22a1 (red) and col1a1 (green) at 3 dpf, which commonly marks connective tissue. Expression was analyzed by HCR. In the transverse confocal sections of the cranial tissue (ventral view, A-F), col22a1 expression is apparent in the periocular tissue (arrowheads, C), craniofacial muscle, including sternohyoideus (sh) and hyohyoideus (hh) muscles, parasphenoid bone (Ps), the heart (h), presumptive chondrocytes within ceratobranchial (cb) and ceratohyal arches (ch), and connective tissue within the developing ear (otic capsule, oc). Note that the expression of both markers overlaps within the parasphenoid bone, otic tissue, connective tissue around the eyes (arrowheads, C), and the tendon/ligament attachment sites of the sternohyoideus and hyohyoideus muscles (arrows, C). Within the myotendinous junction in the trunk region (G-I), all col22a1-expressing cells were also positive for col1a1 expression. Note that col1a1 also labels keratinocytes within the epidermis. (J-L) col22a1 expression analyzed by HCR partially overlaps with vascular endothelial expression of kdrl:GFP. Cranial vasculature of whole-mounted embryos at 3 dpf was imaged dorsally by confocal microscopy. Anterior is to the top. Maximal-intensity projection of selected slices is shown. (M-O) Longitudinal cranial sections of embryos stained by WISH/immunofluorescence for col22a1 (red) and vascular endothelial fli1:GFP (green) at 72 hpf. col22a1 expression is apparent within the perivascular stromal cells (arrows, K). b, brain; e, eye; ec, endothelial cells. Note that these sections are located more ventrally compared with the vascular col22a1 expression shown in J-L.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col1a1a AB standard conditions Protruding-mouth epidermis keratinocyte ISH
Protruding-mouth eye connective tissue cell ISH
Protruding-mouth hyohyoideus muscle tendon junction ISH
Protruding-mouth inner ear connective tissue cell ISH
Protruding-mouth myotome muscle tendon junction ISH
Protruding-mouth parasphenoid ISH
Protruding-mouth sternohyoid muscle tendon junction ISH
col22a1 AB standard conditions Protruding-mouth ceratobranchial cartilage chondrocyte ISH
Protruding-mouth ceratohyal cartilage chondrocyte ISH
Protruding-mouth eye connective tissue cell ISH
Protruding-mouth heart ISH
Protruding-mouth hyohyoideus ISH
Protruding-mouth hyohyoideus muscle tendon junction ISH
Protruding-mouth inner ear connective tissue cell ISH
Protruding-mouth myotome muscle tendon junction ISH
Protruding-mouth parasphenoid ISH
Protruding-mouth sternohyoid ISH
Protruding-mouth sternohyoid muscle tendon junction ISH
s843Tg standard conditions Protruding-mouth cranial vasculature blood vessel endothelium ISH
y1Tg standard conditions Protruding-mouth cranial vasculature stromal cell ISH
EGFP s843Tg standard conditions Protruding-mouth cranial vasculature blood vessel endothelium IHC
y1Tg standard conditions Protruding-mouth cranial vasculature blood vessel endothelium IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.