ZFIN Figure: Carmona-Aldana et al., 2018, Fig. 4

ZFIN ID: ZDB-FIG-190328-34

ZFIN ID: ZDB-FIG-190328-34

Carmona-Aldana et al., 2018 - CTCF knockout reveals an essential role for this protein during the zebrafish development. Mechanisms of Development 154:51-59 Full text @ Mech. Dev.

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	WT + MO3-ctcf WT + MO4-ctcf
Knockdown Reagents:	MO3-ctcf MO4-ctcf
Observed In:	brain segmentation eye forebrain hindbrain midbrain notochord post-vent region somite whole organism
Stage:	Prim-5

Fig. 4 Phenotype of the CTCF morphants. 1-cell to 2-cell embryos were injected with mo-aug or mo-sp. A range of phenotypes was found at 24 hpf, from wild type, mild and severe phenotypes. Different structures were affected: a. Forebrain, b. Midbrain, c. Hindbrain, d. Eye, e. Notochord f. Somites. The percentages and the median of embryos with a mild or severe phenotype are shown in the graph. The statistical difference is noted with an asterisk (n = 4 assays, p = 0.006 for mo-aug; n = 9 assays, p = 0.2609 for mo-sp; one-tailed unpaired t-tests).

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO3-ctcf	standard conditions	Prim-5	brain segmentation decreased process quality, abnormal
		Prim-5	eye hypoplastic, abnormal
		Prim-5	forebrain morphology, abnormal
		Prim-5	hindbrain morphology, abnormal
		Prim-5	midbrain morphology, abnormal
		Prim-5	notochord morphology, abnormal
		Prim-5	post-vent region decreased size, abnormal
		Prim-5	somite morphology, abnormal
		Prim-5	whole organism decreased length, abnormal
WT + MO4-ctcf	standard conditions	Prim-5	brain segmentation decreased process quality, abnormal
		Prim-5	eye hypoplastic, abnormal
		Prim-5	forebrain morphology, abnormal
		Prim-5	hindbrain morphology, abnormal
		Prim-5	midbrain morphology, abnormal
		Prim-5	notochord morphology, abnormal
		Prim-5	somite morphology, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Mechanisms of Development, 154, Carmona-Aldana, F., Zampedri, C., Suaste-Olmos, F., Murillo-de-Ozores, A., Guerrero, G., Arzate-Mejía, R., Maldonado, E., Navarro, R., Chimal-Monroy, J., Recillas-Targa, F., CTCF knockout reveals an essential role for this protein during the zebrafish development, 51-59, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 154, Carmona-Aldana, F., Zampedri, C., Suaste-Olmos, F., Murillo-de-Ozores, A., Guerrero, G., Arzate-Mejía, R., Maldonado, E., Navarro, R., Chimal-Monroy, J., Recillas-Targa, F., CTCF knockout reveals an essential role for this protein during the zebrafish development, 51-59, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.