ZFIN ID: ZDB-FIG-190328-34
Carmona-Aldana et al., 2018 - CTCF knockout reveals an essential role for this protein during the zebrafish development. Mechanisms of Development   154:51-59 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 Phenotype of the CTCF morphants. 1-cell to 2-cell embryos were injected with mo-aug or mo-sp. A range of phenotypes was found at 24 hpf, from wild type, mild and severe phenotypes. Different structures were affected: a. Forebrain, b. Midbrain, c. Hindbrain, d. Eye, e. Notochord f. Somites. The percentages and the median of embryos with a mild or severe phenotype are shown in the graph. The statistical difference is noted with an asterisk (n = 4 assays, p = 0.006 for mo-aug; n = 9 assays, p = 0.2609 for mo-sp; one-tailed unpaired t-tests).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-ctcf standard conditions Prim-5 brain segmentation decreased process quality, abnormal
Prim-5 eye hypoplastic, abnormal
Prim-5 forebrain morphology, abnormal
Prim-5 hindbrain morphology, abnormal
Prim-5 midbrain morphology, abnormal
Prim-5 notochord morphology, abnormal
Prim-5 post-vent region decreased size, abnormal
Prim-5 somite morphology, abnormal
Prim-5 whole organism decreased length, abnormal
WT + MO4-ctcf standard conditions Prim-5 brain segmentation decreased process quality, abnormal
Prim-5 eye hypoplastic, abnormal
Prim-5 forebrain morphology, abnormal
Prim-5 hindbrain morphology, abnormal
Prim-5 midbrain morphology, abnormal
Prim-5 notochord morphology, abnormal
Prim-5 somite morphology, abnormal
Acknowledgments:
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Reprinted from Mechanisms of Development, 154, Carmona-Aldana, F., Zampedri, C., Suaste-Olmos, F., Murillo-de-Ozores, A., Guerrero, G., Arzate-Mejía, R., Maldonado, E., Navarro, R., Chimal-Monroy, J., Recillas-Targa, F., CTCF knockout reveals an essential role for this protein during the zebrafish development, 51-59, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.