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ZFIN ID: ZDB-FIG-181127-23
Mochizuki et al., 2018 - Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish. Development (Cambridge, England)   145(20) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Ectopic lens fiber differentiation occurs in rw341 mutant lens epithelium. (A) Wild-type and rw341 mutant embryos. (B,C) Wild-type (B) and rw341 mutant (C) heads. (D,E) Retinas of wild type (D) and rw341 mutants (E). rw341 mutants show a small lens fiber core, which is surrounded by many aggregated lens cells. (F,G) Higher magnification of the lenses in D and E. (H,H′) Labeling of lenses using anti-Pax6 and PCNA antibodies. In wild type, lens epithelial cells express Pax6 and PCNA (H′, filled arrowheads). Open arrowheads in H′ indicate cells with relatively weak PCNA expression. In multilayered lens epithelium of rw341 mutants (H′, le), most cells express PCNA, but Pax6 expression is weak. (I,I′) Labeling of lenses using anti-Pax6 and BrdU antibodies. Arrows indicate BrdU signals. (J) Percentage of BrdU-positive cells among Pax6-positive lens epithelial cells. Data are mean±s.d. No statistical difference between wild type and rw341 mutants (Student's t-test). (K,K′) Labeling of lenses using anti-Prox1 and PCNA antibodies. In wild type, Prox1 is not expressed in lens epithelium (K′, open arrowheads) and only in early differentiating lens fiber cells (K, arrows). In rw341 mutants, many lens epithelial cells (K′, le) express Prox1 (K′, filled arrowheads). (L,L′) Labeling of lenses using anti-AQP0 antibody. AQP0 is expressed in elongating lens fiber cells in wild type and rw341 mutants (L′, arrows). AQP0-expressing cells were observed in multilayered rw341 mutant lens epithelium (L′, arrowheads). (M) Schematic drawing of wild-type and rw341 mutant lenses. Pax6 is expressed in lens epithelium, whereas Prox1 and AQP0 are expressed in newly differentiating and later elongating lens fiber cells, respectively. In rw341 mutants, ectopic lens fiber differentiation occurs in multilayered lens epithelium. Areas where lens fiber differentiation occurs and does not occur are indicated by red (+) and blue (−), respectively. All lenses are at 5 dpf. Scale bars: 40 µm in B,C; 20 µm in D-L′.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-mip IHC WT standard conditions Day 5 Not Detected lens anterior region
IHC Day 5 Not Detected lens epithelium
IHC Day 5 lens fiber cell
IHC vps45rw341/rw341 standard conditions Day 5 lens anterior region
IHC Day 5 lens epithelium
IHC Day 5 lens fiber cell
Ab1-pax6 IHC WT standard conditions Day 5 lens epithelium
IHC vps45rw341/rw341 standard conditions Day 5 lens epithelium
Ab2-pcna IHC WT standard conditions Day 5 lens epithelium
IHC vps45rw341/rw341 standard conditions Day 5 lens epithelium
Ab3-prox1 IHC WT standard conditions Day 5 Not Detected lens anterior region
IHC Day 5 lens posterior region
IHC Day 5 Not Detected lens epithelium
IHC Day 5 lens fiber cell
IHC vps45rw341/rw341 standard conditions Day 5 lens anterior region
IHC Day 5 lens epithelium
IHC Day 5 lens fiber cell
Phenotype Details
Fish Conditions Stage Phenotype
vps45rw341/rw341 standard conditions Day 5 lens decreased diameter, abnormal
Day 5 lens anterior region ab3-prox1 labeling increased amount, abnormal
Day 5 lens anterior region ab1-mip labeling increased amount, abnormal
Day 5 lens anterior region ab3-prox1 labeling increased distribution, abnormal
Day 5 lens anterior region ab1-mip labeling increased distribution, abnormal
Day 5 lens anterior region ab1-mip labeling mislocalised, abnormal
Day 5 lens anterior region ab3-prox1 labeling mislocalised, abnormal
Day 5 lens epithelium ab1-pax6 labeling decreased amount, abnormal
Day 5 lens epithelium ab2-pcna labeling increased amount, abnormal
Day 5 lens epithelium ab2-pcna labeling increased distribution, abnormal
Day 5 lens epithelium increased thickness, abnormal
Day 5 lens epithelium ab3-prox1 labeling mislocalised, abnormal
Day 5 lens epithelium ab1-mip labeling mislocalised, abnormal
Day 5 lens fiber cell differentiation process quality, abnormal
Day 5 lens fiber cell morphogenesis process quality, abnormal
Day 5 lens mitotic cell cycle occurrence, normal
Day 5 retina retina layer formation process quality, normal
Day 5 swim bladder uninflated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development