FIGURE

Fig. 2

ID
ZDB-FIG-181101-8
Publication
Fernandez et al., 2018 - RES complex is associated with intron definition and required for zebrafish early embryogenesis
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Fig. 2

RES complex is required during zebrafish brain development.

(A) Acridine orange (ao) staining of zebrafish mutant embryos for bud13 (30–32 hpf), rbmx2 and snip1 (48 hpf). Mutants show a marked degree of cells with nuclear uptake of ao compared to WT sibling most predominantly in the head. WT: represent phenotypically wild type sibling from the same mutant fish line. (B) Maximum intensity projections of individual and merged channels (GFP and dsRed) of 3D confocal images of bud13Δ7/Δ7 and their wild-type siblings (scale bar 20 μm) in transgenic lines that label GABAergic neurons and precursors (Tg[dlx6a-1.4kbdlx5a/ dlx6a:GFP]) and glutamatergic neurons (Tg[vglut:DsRed]). WT: represent phenotypically wild type sibling from the same mutant fish line. (C) Total number of dlx5a/6a:GFP+ cells (GABAergic neurons and precursors) and vglut:DsRed+ cells (glutamatergic neurons) in the forebrain of the bud13Δ7/Δ7 (n = 3) and WT sibling (n = 4) were quantified. *** vglut: P = 2 x 10−4; ***dlx5a/6a: P = 3 x 10−4 (one-way ANOVA).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Phenotype Detail
Acknowledgments
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