header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-181101-7
Fernandez et al., 2018 - RES complex is associated with intron definition and required for zebrafish early embryogenesis. PLoS Genetics   14:e1007473 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: High to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 1

RES complex is essential for early vertebrate development.

(A) Schematic model of the RES complex adapted from Brooks et al. [8]. Rbmx2 (light blue) is the core subunit with an RRM-domain structure. Bud13 (orange) and Snip1 (pink) interact with Rbmx2 (light blue). (B) In situ hybridization showing spatial and temporal expression of RES complex members. (C-E) Gene models of the mutant allele generated using CRISPR-Cas9-nanos. (C) bud13, 7 nt deletion in exon 6 generated a premature stop codon. (D) rbmx2, 16 nt deletion removed exon-intron boundary at exon 2 (exon capital letter, intron lower letter). (E) snip1, 11 nt deletion in exon 1 generated a premature stop codon. f-g) Lateral view of RES complex mutant embryos, their corresponding WT sibling and mutants injected with the cognate mRNA. (F) bud13 mutant at 32 hpf (scale bar: 0.35mm) and at 48 hpf (scale bar: 0.5mm). (G, H) rbmx2 and snip1 mutant at 48 hpf respectively. WT: represent phenotypically wild type sibling from the same mutant fish line.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bud13 WT standard conditions High blastodisc ISH
Prim-5 central nervous system ISH
rbmx2 WT standard conditions High blastodisc ISH
Prim-5 central nervous system ISH
snip1 WT standard conditions High blastodisc ISH
Prim-5 central nervous system ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bud13ya103/ya103 standard conditions Prim-15 brain morphology, abnormal
Prim-15 central nervous system cell death increased occurrence, abnormal
Long-pec brain morphology, abnormal
Long-pec central nervous system cell death increased occurrence, abnormal
rbmx2ya102/ya102 standard conditions Long-pec brain morphology, abnormal
Long-pec central nervous system cell death increased occurrence, abnormal
snip1ya104/ya104 standard conditions Long-pec brain morphology, abnormal
Long-pec central nervous system cell death increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.