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ZFIN ID: ZDB-FIG-181022-2
Lawrence et al., 2018 - The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function, which leads to early-onset osteoarthritis.. Philosophical transactions of the Royal Society of London. Series B, Biological sciences   373(1759) Full text @ Phil. Trans. Roy. Soc. Lond., Series B
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Days 7-13

Fig. 1

col11a2 zebrafish mutant larvae show progressively altered type II collagen protein localization in jaw cartilage. (a,b) Maximum projection of ventral and lateral confocal image stacks from wt (a) and homozygous mutant (col11a2−/−) (b) larvae immunostained for type II collagen at three time points (3, 5 and 7 dpf). White arrows indicate areas of change in type II collagen distribution in the ECM. Dashed insets show single-stack images of regions with reduced deposition (white asterisks represent areas where type II collagen is maintained in mutant fish and red asterisks show fragments of type II collagen-positive material outside the main cartilage elements). Red arrows show interoperculomandibular (IOM) ligament. Scale bar, 100 µm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Protruding-mouth ventral mandibular arch
IHC Day 5 ventral mandibular arch
IHC Days 7-13 ventral mandibular arch
IHC col11a2sa18324/sa18324 standard conditions Protruding-mouth ventral mandibular arch
IHC Day 5 interopercular-mandibular ligament
IHC Day 5 ventral mandibular arch
IHC Days 7-13 ventral mandibular arch
Phenotype Details
Fish Conditions Stage Phenotype
col11a2sa18324/sa18324 standard conditions Prim-5 ventral mandibular arch morphology, abnormal
col11a2sa18324/sa18324 standard conditions Day 5 interopercular-mandibular ligament ab1-col2a labeling mislocalised, abnormal
col11a2sa18324/sa18324 standard conditions Day 5 ventral mandibular arch ab1-col2a labeling decreased amount, abnormal
col11a2sa18324/sa18324 standard conditions Days 7-13 ventral mandibular arch ab1-col2a labeling decreased amount, abnormal
col11a2sa18324/sa18324 standard conditions Days 7-13 ventral mandibular arch morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Philosophical transactions of the Royal Society of London. Series B, Biological sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Phil. Trans. Roy. Soc. Lond., Series B