FIGURE

Fig. 5

ID
ZDB-FIG-181004-5
Publication
Wu et al., 2018 - Il34-Csf1r Pathway Regulates the Migration and Colonization of Microglial Precursors
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Il34-Csf1ra Pathway and Neuronal Apoptosis Collaboratively Orchestrate Microglia Colonization

(A and B) Representative images (A) and quantification (B) of microglia in the optic tectum of 3-dpf WT (n = 8), Tg(Xla.Tubb:il34) (n = 6), Tg(Xla.Tubb:bcl-2) (n = 13), and Tg(Xla.Tubb:il34;Xla;Tubb:bcl-2) (n = 5) embryos. Microglia were labeled by Tg(mpeg1:DsRedx) (red). The optic tectum is indicated by dashed lines. Values represent means with SD.

(C and D) WISH showing mfap4 expression (C) and quantification (D) of mfap4+ macrophages in the head region of 30-hpf il34−/− embryos or Tg(Xla.Tubb:bcl-2);il34−/− embryos. n = 19 and 16 for il34−/− and Tg(Xla.Tubb:bcl-2);il34−/− embryos, respectively. Values represent means ± SD.

(E and F) Representative transverse sections through the midbrain and hindbrain of 3-dpf WT or Tg(Xla.Tubb:bcl-2);il34−/− embryos (E), with macrophages labeled by Tg(mpeg1:DsRedx) (red), neurons labeled by HuC/D immunostaining (green), and quantification of DsRedx+ microglia in the whole CNS (F). The CNS region is indicated by dashed lines. n = 6, 11, 6, and 8 for WT, Tg(Xla.Tubb:bcl-2), il34−/−, and Tg(Xla.Tubb:bcl-2);il34−/− embryos, respectively. Values represent means with SD.

ns, p > 0.05; p ≤ 0.05; ∗∗∗∗p ≤ 0.0001.

Expression Data
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 46, Wu, S., Xue, R., Hassan, S., Nguyen, T.M.L., Wang, T., Pan, H., Xu, J., Liu, Q., Zhang, W., Wen, Z., Il34-Csf1r Pathway Regulates the Migration and Colonization of Microglial Precursors, 552-563.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell