FIGURE

Fig. 5

ID
ZDB-FIG-180918-6
Publication
Lisowska et al., 2018 - Cerebral Cavernous Malformation 1/2 complex controls ROCK1 and ROCK2 complementary functions for endothelial integrity
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Fig. 5

ROCK1 silencing in ccm1 zebrafish mutant embryos rescues cardiac morphogenesis. (A–J) Endocardial cells, marked by Tg(kdrl:EGFP) (inverted images) at 48 hpf. Details with single confocal plane section of the atrioventricular canal (AVC) are shown in A′–J′. Scale bars: 30 µm (main images); 10 µm (detailed images). (K) Quantification of the number of embryos that showed a phenotypic rescue of the krit1/ccm1ty219c cardiac phenotype at 48 hpf. Embryos from krit1/ccm1ty219c/+ incrosses were 75% phenotypically WT (blue bars), while 25% showed krit1/ccm1ty219c mutant phenotype of cardiac ballooning (white bars). Embryos from krit1/ccm1ty219c/+ incrosses that were injected with high doses of rock1-MO1 and -MO2 showed a rescue of the cardiac ballooning (red), whereas embryos injected with low MO doses failed to show significant numbers with rescue. However, when combined, low doses of rock1-MO1 and -MO2 rescued the cardiac ballooning phenotype in krit1/ccm1ty219c mutant embryos as efficiently as single high dose MOs. Total numbers quantified, from a set of three independent zebrafish cohorts, are n=195 for control embryos; rock1-MO1 injected at high dose, n=232; rock1-MO2 at high dose, n=126; rock1-MO1 at low dose, n=167; rock1-MO2 at low dose, n=148; and combined rock1-MO1 and MO2 at low dose, n=134. *P<0.05 (two-way ANOVA with Dunett's multiple comparisons post-test). Embryos from krit1/ccm1ty219c/+ incrosses that were injected with either rock2a-MO1 or rock2b-MO2 did not rescue the cardiac ballooning phenotype, however, a fraction of the sibling population show MO-specific defects that are similar to the krit1/ccm1ty219c phenotype. Control for rock2a-MO, n=230; rock2a-MO, n=265; control for rock2b-MO, n=526; rock2b-MO, n=317.

Expression Data
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.