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ZFIN ID: ZDB-FIG-180914-22
Rasmussen et al., 2018 - Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization. Developmental Cell   46(3):344-359.e4 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 6

Osteoblasts, but Not Blood Vessels, Promote Epidermal Re-innervation following Injury

(A) Regeneration of a single scale and associated axons. Black box, region of images in (A′)–(A‴). Green boxes, regions of magnification in lower panels. Red dashed lines, scale margins before and after removal. Blue dashed lines, margins of non-injured scales. Note that regenerating axons developed growth cones in the dermis at 1 dpi (arrowheads), sparsely innervated the epidermis at 2 dpi, and more extensively innervated the epidermis at 7 dpi.

(B) Experimental design for scale regeneration in the presence of 500 nM PTK787.

(C and D) Boxplots of vessel (C) and axon (D) density based on Tg(fli1a:EGFP) expression or acTubulin staining, respectively, in regenerating scales at 21 dpi. n = 31–44 scales from n = 2–3 fish. p < 0.01; n.s., not significant (p > 0.01); Wilcoxon rank-sum test.

(E and F) Representative lateral views of the indicated markers in control (DMSO) or VEGFR inhibitor (PTK787)-treated skin. Note that neither treatment prevented the formation or maintenance of nerves (arrows) over the course of the experiment. Dashed lines, scale margins.

(G) Timeline of osteoblast ablation using metronidazole (Mtz).

(H–J) Quantification (H) and representative lateral views and tracings (I and J) of regenerating axons in control or osteoblast-ablated fish (Mtz) at 7 dpi. Axon length is expressed as millimeters per 0.0625 mm2 region of skin. n = 9–12 regions/treatment from n = 2–3 fish. Black bars, mean ± SEM. p < 0.01, Wilcoxon rank-sum test.

Transgenes: (A) axons (Tg(p2rx3a>mCherry)); (E and F) axons (Tg(p2rx3a>mCherry)) and endothelium (Tg(fli1a:EGFP)); (I and J) axons (Tg(p2rx3b:EGFP)). Scale bars, 50 μm (A, I, and J) and 200 μm (E and F). See also Figure S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry la207Tg ablation: scale Adult regenerating tissue axon IFL
Adult scale axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: vatalanib succinate, ablation: scale Adult scale axon regeneration occurrence, normal
sl1Tg ; pd46Tg chemical ablation: osteoblast, chemical treatment by environment: metronidazole, ablation: scale Adult regenerating tissue axon decreased length, abnormal
Adult regenerating tissue axon regeneration decreased occurrence, abnormal
Adult scale axon regeneration decreased occurrence, abnormal
y1Tg chemical treatment by environment: vatalanib succinate, ablation: scale Adult regenerating tissue vasculature morphology, abnormal
Adult regenerating tissue vasculature development decreased occurrence, abnormal
Adult scale axon regeneration occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 46(3), Rasmussen, J.P., Vo, N.T., Sagasti, A., Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization, 344-359.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell