FIGURE

Fig. S5

ID

ZDB-FIG-180823-41

Publication

Ma et al., 2018 - Identification of DEAD-Box RNA Helicase DDX41 as a Trafficking Protein That Involves in Multiple Innate Immune Signaling Pathways in a Zebrafish Model

Other Figures

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Fig. S5

Subcellular localization analysis of DrDDX41 in zebrafish WBCs and ZF4 cells. (A) Western blot shows that the rabbit anti-DrDDX41 Ab can specifically bind to the corresponding endogenous target proteins in the whole cell lysates of the WBCs sorted from the peritoneal blood, kidney, and spleen. (B) Confocal microscopy image of WBCs stained with rabbit anti-DrDDX41 Ab, showing that the DrDDX41 protein partially distributed in nucleus and cytoplasm in the natural WBCs. WBCs stained with a non-related rabbit IgG was used as negative control. (C) Representative images of transfected ZF4 cells with pEGFP-N1 and pEGFP-DrDDX41. Nuclei were stained with the DNA-intercalating dye DAPI. Scale bars represent 5 μm. Images were captured under a laser scanning confocal microscope (Zeiss LSM-710; original magnification, 630×).

Expression Data

Gene:	ddx41
Antibody:	Ab1-ddx41
Fish:	AB
Anatomical Terms:	leukocyte cytoplasm leukocyte nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol