header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180730-13
Than-Trong et al., 2018 - Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signaling cascade in the vertebrate adult brain. Development (Cambridge, England)   145(10) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Adult

Fig. 6

Hey1-depleted RGs lose stemness characters. (A-D) Effect of Hey1 abrogation on Sox2 expression in adult RGs. (A) Experimental scheme: fluorescein-tagged MOs (control MO or hey1 splice MO) are electroporated into the pallial VZ and Sox2 expression is analysed after 3 days. (B,C) Examples of electroporated VZ double-immunostained for Gs (white) and Sox2 (magenta), with MO-containing cells in green. Colour-coded arrows indicate the different cell types (see D). (D) Quantification of the proportion of Sox2-positive (blue) and -negative (green) RGs within MO-electroporated cells. (E-J) Effect of Hey1 abrogation on RG reactivation potential. (E) Experimental scheme: fluorescein-tagged MOs are electroporated into the pallial VZ and LY411575 (or the vehicle DMSO) is applied into the swimming water between 2 and 4 days post-electroporation. RG proliferation is analysed at 4 days. (F-I) Representative examples of whole-mount electroporated/LY-treated brains double immunoprocessed for Gs (white) and PCNA (magenta). (J) Quantification of the proportions of the different cell types (colour-code indicated in E). LY treatment induces RG activation (decrease in the proportion of qRGs, increase in the proportion of aRGs) upon electroporation of the control MO, but is without effect when Hey1 function is abrogated. Number of cells counted per brain: 99-293 for control MO treated with DMSO; 153-298 for splice MO treated with DMSO; 98-262 for control MO treated with LY; and 151-353 for splice MO treated with LY. n=3-5 brains per condition. *P<0.05, **P<0.01, ***P<0.001, all pairwise comparisons were adjusted for multiple comparisons following the Holm's procedure. Scale bars: 10 µm in B,C; 70 µm in F-I.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC AB control Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB chemical treatment by environment: LY-411575 Adult dorsal telencephalon radial glial cell
IHC Adult telencephalon radial glial cell
IHC AB + MO3-hey1 control Adult dorsal telencephalon radial glial cell
IHC Adult telencephalon radial glial cell
IHC AB + MO3-hey1 standard conditions Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB + MO3-hey1 chemical treatment by environment: LY-411575 Adult dorsal telencephalon radial glial cell
IHC Adult telencephalon radial glial cell
Ab2-pcna IHC AB control Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB chemical treatment by environment: LY-411575 Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB + MO3-hey1 control Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB + MO3-hey1 chemical treatment by environment: LY-411575 Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
Ab3-sox2 IHC AB control Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
IHC AB + MO3-hey1 standard conditions Adult dorsal telencephalon radial glial cell
IHC Adult dorsal telencephalon ventricular zone
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: LY-411575 Adult radial glial cell stem cell proliferation increased occurrence, abnormal
AB + MO3-hey1 standard conditions Adult radial glial cell neuronal stem cell decreased amount, abnormal
AB + MO3-hey1 chemical treatment by environment: LY-411575 Adult radial glial cell stem cell proliferation occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development