FIGURE

Fig. 4

ID
ZDB-FIG-180705-57
Publication
Williams et al., 2018 - Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes
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Fig. 4

Gon4l regulates axis extension independent of PCP signaling. ad Live embryos at 24 hpf resulting from a cross between a germline-replaced udu−/−;kny fr6/+ female and an udu+/-;knyfr6/+ male. Genotypes are indicated in the upper right corner, fractions indicate the number of embryos in the clutch with the pictured phenotype. e Mosaically expressed Prickle (Pk)-GFP in WT and MZudu−/− gastrulae. Arrowheads indicate anteriorly localized Pk-GFP puncta. Membrane Cherry marks cell membranes, nuclear-RFP marks cells injected with pk-gfp RNA. Images are representative of three independent experiments. f Quantification of Pk-GFP localization shown in e (chi-square, p = 0.07). g Quantification of notochord boundary straightness in WT and kny−/− gastrulae. Symbols are means with SEM (two-way ANOVA, ****p < 0.0001). h, i, l, m Still images from live time-lapse confocal movies of the axial mesoderm in kny−/− (hi) and kny−/−;udu−/− (lm) gastrulae at the time points indicated. Cell outlines are colored as in Fig. 3. j, n Quantification of axial mesoderm cell orientation as in Fig. 3, bars are median values (Kolmogorov–Smirnov test, ***p = 0.0003). k, o Quantification of axial mesoderm cell elongation as in Fig. 3, bars are mean values. N indicates number of embryos analyzed (and number of cells in f). Scale bar is 500 μm in ad, 10 μm in e, 50 μm in hm

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: 75%-epiboly to Prim-5

Phenotype Detail
Acknowledgments
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