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ZFIN ID: ZDB-FIG-180508-37
König et al., 2017 - Dynamics of actinotrichia regeneration in the adult zebrafish fin. Developmental Biology   433(2):416-432 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5

Elongated uninjured fins of alfdty86 mutant fish contain extended actinotrichia. (A, B) Live-imaging of adult caudal fins in wild-type fish and alfdty86 mutants. (C, D) Alcian blue histological staining of uninjured caudal fins shows uncalcified lepidotrichia/cartilage (blue). Bundles of actinotrichia are unstained, but visible by a differential contrast at the tips of rays. Brown patches correspond to endogenous fin pigmentation. N = 4 fins. (E, F) Double immunostaining of wild-type and alfdty86 mutant fins using And1 (green) and osteoblast marker Zns5 (red) antibodies. Tissue autofluorescence shown in blue. N = 4 fins. (G, H) In-situ hybridization with and1 probe on whole-mount fins labels uninjured fin margin of alfdty86 mutant fins, but not wild-type ones. N = 5 fins. (I) Quantification of length of actinotrichia in wild-type versus alfdty86 uninjured fins based on And1 staining (6 rays quantified for each fin, N = 4 fish per group. Error bars indicate SEM, ** P<0.01).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
and1 WT control Adult Not Detected caudal fin anatomical margin ISH
kcnk5bty86d/ty86d control Adult caudal fin anatomical margin ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-and1 IHC WT control Adult caudal fin anatomical margin
IHC kcnk5bty86d/ty86d control Adult caudal fin anatomical margin
zns-5 IHC WT control Adult caudal fin osteoblast
IHC kcnk5bty86d/ty86d control Adult caudal fin osteoblast
Phenotype Details
Fish Conditions Stage Phenotype
kcnk5bty86d/ty86d control Adult caudal fin actinotrichium increased length, abnormal
Adult caudal fin anatomical margin and1 expression increased distribution, abnormal
Adult caudal fin anatomical margin ab2-and1 labeling increased distribution, abnormal
kcnk5bty86d/ty86d amputation: caudal fin Adult caudal fin actinotrichium fin regeneration increased efficacy, abnormal
Adult regenerating fin caudal fin actinotrichium increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 433(2), König, D., Page, L., Chassot, B., Jaźwińska, A., Dynamics of actinotrichia regeneration in the adult zebrafish fin, 416-432, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.