ZFIN ID: ZDB-FIG-180420-56
Raghupathy et al., 2017 - Rpgrip1 is required for rod outer segment development and ciliary protein trafficking in zebrafish. Scientific Reports   7:16881 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 14-20 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 14-20 to Adult

Fig. 5

Immunostaining with Zpr1 antibody exhibited red/green double cones that were normal at early ages and degenerated at later ages. Nuclei were shown in blue using DAPI. Double cones of rpgrip1 −/− mutants were morphologically normal at 2wpf and 1 mpf, shown here. While at 3mpf, double cones were disorganized, the number of double cones was markedly reduced at 6 mpf and only some double cone cell bodies remained at 12 mpf.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Days 14-20 retinal cone cell
IHC Days 14-20 retinal photoreceptor layer green sensitive photoreceptor cell
IHC Days 14-20 retinal photoreceptor layer red sensitive photoreceptor cell
IHC Days 30-44 retinal cone cell
IHC Days 30-44 retinal photoreceptor layer green sensitive photoreceptor cell
IHC Days 30-44 retinal photoreceptor layer red sensitive photoreceptor cell
IHC Adult retinal cone cell
IHC Adult retinal photoreceptor layer green sensitive photoreceptor cell
IHC Adult retinal photoreceptor layer red sensitive photoreceptor cell
IHC rpgrip1fh314/fh314 standard conditions Days 14-20 retinal cone cell
IHC Days 14-20 retinal photoreceptor layer green sensitive photoreceptor cell
IHC Days 14-20 retinal photoreceptor layer red sensitive photoreceptor cell
IHC Days 30-44 retinal cone cell
IHC Days 30-44 retinal photoreceptor layer green sensitive photoreceptor cell
IHC Days 30-44 retinal photoreceptor layer red sensitive photoreceptor cell
IHC Adult retinal cone cell
IHC Adult retinal photoreceptor layer green sensitive photoreceptor cell
IHC Adult retinal photoreceptor layer red sensitive photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
rpgrip1fh314/fh314 standard conditions Days 14-20 retinal cone cell organized, normal
Days 30-44 retinal cone cell organized, normal
Adult retinal cone cell disorganized, abnormal
Adult retinal photoreceptor layer has fewer parts of type retinal cone cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.