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ZIRC
ZFIN ID: ZDB-FIG-180418-8
Goody et al., 2017 - Influenza A Virus Infection Damages Zebrafish Skeletal Muscle and Exacerbates Disease in Zebrafish Modeling Duchenne Muscular Dystrophy. PLoS Currents   9 Full text @ PLoS Curr.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 2 IAV infection compromises the sarcolemma All embryo images are side mounts, dorsal top, anterior left. (A) Tg(fli1:GFP) zebrafish embryo with labeled endothelial cells (green) DC-injected with PBS plus EBD (red). EBD remains in the vasculature. White arrowheads point to EBD in an intersomitic vessel (top left), the dorsal aorta (middle), and the caudal vein (bottom right). (B-C) Wild-type zebrafish injected with PBS plus EBD. (B) Cropped EBD panel. (B1) Cropped EBD and brightfield panels merged. (C) EBD panel. Note that muscle fibers are impermeable to EBD in PBS-injected zebrafish. (D) Tg(fli1:GFP) zebrafish embryo DC-injected with IAV plus EBD (red). EBD leaked out of the vasculature and penetrated muscle fibers (black arrowheads). (E-F) Wild-type zebrafish injected with IAV plus EBD. (E) Cropped EBD panel. (E1) Cropped EBD and brightfield panels merged. (F) EBD panel. Note the uptake of EBD by long (black arrowheads) and retracted fibers (white arrowheads) indicative of sarcolemma damage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Protruding-mouth vasculature endothelial cell IFL
y1Tg viral treatment by injection: unidentified influenza virus Protruding-mouth vasculature endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg viral treatment by injection: unidentified influenza virus Protruding-mouth skeletal muscle cell detached from skeletal muscle cell, abnormal
Protruding-mouth skeletal muscle sarcolemma broken, abnormal
Protruding-mouth vasculature increased permeability, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Currents for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Curr.