ZFIN ID: ZDB-FIG-180308-9
Zhang et al., 2017 - Endoderm Jagged induces liver and pancreas duct lineage in zebrafish. Nature communications   8:769 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 4

Intrahepatopancreatic ducts do not contribute significantly to the developing acinar and hepatocyte lineages. ad Immunofluorescence analysis of the pancreas (a, b) and liver (c, d) in 5 dpf wild type (a, c) and jag1b −/− ;jag2b −/− mutant embryos (b, d, bottom panels with lfabp10:DsRed channel removed). Despite the absence of ductal cells in jag1b −/− ;jag2b −/− mutants b, d, lumen formation (arrows) and apical localization of ZO-1 (red in b, green in d) remain comparable to wild type siblings (a, c). Normal differentiation indicated by zymogen granule accumulation in the ptf1a:GFP+ pancreatic acinar cells (green) and lfabp-DsRed expression (red) in hepatocytes. e Schematic of approach to Notch activity lineage tracing. fi Notch active labeled H2B-mCherry+ cells (red) in the liver are all Prox1+ (blue) and Hnf4a (green) (n = 12 embryos, 1,416 labeled cells counted from two different experiments (f), P < 0.0001). g, h In the pancreas, nearly all H2B-mCherry+ cells (red) co-express Nkx6.1 (blue) and are ptf1a:GFP (green) (n = 24 embryos, 1,586 labeled cells counted from 3 different experiments, P < 0.0001) g Only a few H2B-mCherry+ cells that lack Nkx6.1 but are ptf1a:GFP+ were observed (arrows, h. n = 4/24 embryos, 12 cells total). i Graph summarizing numbers of Notch active labeled H2B-mCherry+ cells found in hepatocytes versus intrahepatic duct (IHD) cells and acinar pancreas versus intrapancreatic duct (IPD) cells. Representative samples; ad, n = 4 each. Scale bars 25 μM

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg standard conditions Day 5 liver hepatocyte IHC
gz15Tg ; jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Day 5 liver hepatocyte IHC
EGFP jh1Tg standard conditions Day 5 Not Detected intrapancreatic duct IHC
Day 5 pancreatic acinar cell IHC
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Day 5 pancreatic acinar cell IHC
jh63Tg ; s959Tg ; jh1Tg chemical treatment by environment: tamoxifen Day 4 Not Detected intrapancreatic duct IHC
Day 4 pancreatic acinar cell IHC
hnf4a ab1-hnf4a jh63Tg ; s959Tg chemical treatment by environment: tamoxifen Day 4 Not Detected intrahepatic bile duct IHC
mCherry jh63Tg ; s959Tg chemical treatment by environment: tamoxifen Day 4 intrahepatic bile duct IHC
jh63Tg ; s959Tg ; jh1Tg chemical treatment by environment: tamoxifen Day 4 intrapancreatic duct IHC
nkx6.1 Ab2-nkx6 jh63Tg ; s959Tg ; jh1Tg chemical treatment by environment: tamoxifen Day 4 intrapancreatic duct IHC
prox1a Ab3-prox1 gz15Tg standard conditions Day 5 liver hepatocyte IHC
Ab3-prox1 gz15Tg ; jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Day 5 liver hepatocyte IHC
Ab3-prox1 jh63Tg ; s959Tg chemical treatment by environment: tamoxifen Day 4 intrahepatic bile duct IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-tjp1 IHC gz15Tg standard conditions Day 5 hepatocyte apical junction complex
IHC gz15Tg ; jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Day 5 hepatocyte apical junction complex
IHC jh1Tg standard conditions Day 5 pancreatic acinar cell apical junction complex
IHC jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Day 5 pancreatic acinar cell apical junction complex
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg ; jag2bhu3425/hu3425 ; jag1bb1105/b1105 standard conditions Day 5 hepatocyte differentiated, normal
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Day 5 pancreatic acinar cell differentiated, normal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.