ZFIN ID: ZDB-FIG-180308-8
Zhang et al., 2017 - Endoderm Jagged induces liver and pancreas duct lineage in zebrafish. Nature communications   8:769 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Pec-fin to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Pec-fin to Day 5

Fig. 3

Distinct early and late roles of Notch signaling in regulating duct and endocrine lineages. a, b Pancreas of 60 hpf jag1b −/− ;jag2b −/− and wt sibling embryos. Neogenic endocrine cells expressing Islet1 (red) are normally found outside the principal islet at the base of the wild type pancreas (arrows, a). These neogenic endocrine cells are not present in the 60hpf jag1b −/− ;jag2b −/− mutant pancreas (b, representative sample, n = 18, pooled from three different jag1b −/+ ;jag2b −/+ in-cross clutches), which is entirely ptf1a:GFP+ (green) with the exception of the principal islet. c, d Pancreas of 5 dpf-treated embryos. Early inhibition of Notch signaling with gamma secretase inhibitor LY575411 treatment (d, n = 9 and c, n = 5. Samples from 2 different experiments) at 30–72 hpf leads to loss of Nkx6.1+ pancreatic duct (red) without excess neuroD:GFP+ (green) endocrine cells. e, f In contrast, late LY575411 treatment (f, n = 9, and n = 6 for control, e Samples pooled from 2 different experiments) also leads to loss of nkx6.1+ pancreatic duct (red) that is coupled with excess neuroD:GFP+ (green) endocrine cells (arrowheads, f). Scale bar, 50 μM

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP jh1Tg standard conditions Pec-fin pancreatic acinar cell IHC
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Pec-fin pancreatic acinar cell IHC
nl1Tg control Day 5 endocrine pancreas IHC
nl1Tg chemical treatment by environment: LY-411575 Day 5 endocrine pancreas IHC
nkx6.1 Ab2-nkx6 nl1Tg control Day 5 intrapancreatic duct IHC
Ab2-nkx6 nl1Tg chemical treatment by environment: LY-411575 Day 5 Not Detected exocrine pancreas IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-Cadherin IHC jh1Tg standard conditions Pec-fin intrapancreatic duct
IHC jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Pec-fin exocrine pancreas
Ab2-isl IHC jh1Tg standard conditions Pec-fin endocrine pancreas
IHC jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Pec-fin endocrine pancreas
Phenotype Details
Fish Conditions Stage Phenotype
jh1Tg ; jag1bb1105/b1105 ; jag2bhu3425/hu3425 standard conditions Pec-fin endocrine pancreas has fewer parts of type endocrine cell, abnormal
Pec-fin exocrine pancreas ab1-pan-cadherin labeling decreased amount, abnormal
Pec-fin exocrine pancreas ab1-pan-cadherin labeling decreased distribution, abnormal
nl1Tg chemical treatment by environment: LY-411575 Day 5 exocrine pancreas nkx6.1 expression absent, abnormal
Day 5 exocrine pancreas lacks all parts of type intrapancreatic duct, abnormal
Day 5 exocrine pancreas development decreased process quality, abnormal
Day 5 Notch signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.