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General Information
ZIRC
ZFIN ID: ZDB-FIG-180302-21
Zhang et al., 2017 - m6A modulates haematopoietic stem and progenitor cell specification.. Nature   549(7671):273-276 Full text @ Nature
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh11Tg; y1Tg standard conditions Prim-15 endothelial cell IFL
jh11Tg; y1Tg + MO1-mettl3 standard conditions Prim-15 endothelial cell IFL
mCherry jh11Tg; y1Tg standard conditions Prim-15 ventral wall of dorsal aorta IFL
jh11Tg; y1Tg + MO1-mettl3 standard conditions Prim-15 ventral wall of dorsal aorta IFL
notch1a TU standard conditions Prim-15 endothelial cell RTPCR
TU + MO1-mettl3 standard conditions Prim-15 endothelial cell RTPCR
runx1 TU standard conditions Prim-5 whole organism RTPCR
TU + MO1-mettl3 standard conditions Prim-5 whole organism RTPCR
TU + MO1-mettl3 chemical treatment: LY-411575 Prim-5 whole organism RTPCR
TU + MO1-mettl3 + MO1-notch1a standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-notch1 WB TU standard conditions Prim-5 whole organism
WB TU + MO1-mettl3 standard conditions Prim-5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-mettl3 standard conditions Prim-5 whole organism runx1 expression decreased amount, abnormal
Prim-5 whole organism Ab3-notch1 labeling increased amount, abnormal
Prim-15 endothelial cell notch1a expression increased amount, abnormal
TU + MO1-mettl3 chemical treatment: LY-411575 Prim-5 whole organism runx1 expression amount, ameliorated
TU + MO1-mettl3 + MO1-notch1a standard conditions Prim-5 whole organism runx1 expression amount, ameliorated
jh11Tg; y1Tg + MO1-mettl3 standard conditions Prim-15 endothelial cell EGFP expression increased amount, abnormal
Full text @ Nature