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ZIRC
ZFIN ID: ZDB-FIG-180207-25
LeBert et al., 2018 - Damage-induced reactive oxygen species regulate vimentin and dynamic collagen-based projections to mediate wound repair.. eLIFE   7:e30703 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6

Projections contained both SHG fibers and Type II Collagen, and the expression of collagen at wounds was regulated by vimentin and ROS.

(A) SHG and type II collagen fibers tightly associate in unwounded caudal fins and (B) within projections at 24 hpw as indicated by SHG imaging and immunofluorescence as observed in two experiments. (C) Schematic of col1a1 and col2a1b expression analysis from the caudal fin. (D) col1a1 and col2a1b expression increased in the wounded caudal fin by 24 hpw as assessed by qRT-PCR (col1a1 8 hpw p=0.0779, n = 5 replicates; 24 hpw p=0.0153, n = 6 replicates; col2a1b 8 hpw p=0.9491, n = 3 replicates, 24 hpw p=0.0294, n = 5 replicates). (E) Morpholino knockdown of vimentin expression reduced the expression of col1a1 and col2a1b in the wounded caudal fin compared to control (col1a1 p=0.004, n = 6 replicates; col2a1b p=0.0404, n = 5 replicates). (F) Early inhibition of ROS using DPI significantly reduced expression of col1a1 and col2a1b at 24 hpw (col1a1 p=0.0464, n = 4 replicates; col2a1b p=0.0126). *p<0.05. ****p<0.0001; error bars are standard deviation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col1a1a AB standard conditions Protruding-mouth caudal fin RTPCR
AB resection: caudal fin Protruding-mouth regenerating fin caudal fin RTPCR
AB + MO2-vim resection: caudal fin, chemical treatment: dibenziodolium Protruding-mouth regenerating fin caudal fin RTPCR
AB + MO2-vim resection: caudal fin Protruding-mouth regenerating fin caudal fin RTPCR
col2a1b AB standard conditions Protruding-mouth caudal fin RTPCR
AB resection: caudal fin Protruding-mouth regenerating fin caudal fin RTPCR
AB + MO2-vim resection: caudal fin, chemical treatment: dibenziodolium Protruding-mouth regenerating fin caudal fin RTPCR
AB + MO2-vim resection: caudal fin Protruding-mouth regenerating fin caudal fin RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC AB standard conditions Protruding-mouth caudal fin collagen type II trimer
IHC AB resection: caudal fin Protruding-mouth regenerating fin collagen type II trimer
Phenotype Details
Fish Conditions Stage Phenotype
AB resection: caudal fin Protruding-mouth regenerating fin caudal fin col2a1b expression increased amount, abnormal
Protruding-mouth regenerating fin caudal fin col1a1a expression increased amount, abnormal
Protruding-mouth regenerating fin fibrillar collagen trimer disorganized, abnormal
AB + MO2-vim resection: caudal fin, chemical treatment: dibenziodolium Protruding-mouth regenerating fin caudal fin col2a1b expression decreased amount, abnormal
Protruding-mouth regenerating fin caudal fin col1a1a expression decreased amount, abnormal
AB + MO2-vim resection: caudal fin Protruding-mouth regenerating fin caudal fin col2a1b expression decreased amount, abnormal
Protruding-mouth regenerating fin caudal fin col1a1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife