ZFIN ID: ZDB-FIG-180206-36
Bian et al., 2017 - Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signalings in the zebrafish embryo. The Journal of biological chemistry   292(34):14165-14175 Full text @ J. Biol. Chem.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Prim-5

Fig. 4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
clocka Ab1-clock AB control Shield whole organism WB
Ab1-clock AB + MO1-clocka standard conditions Shield whole organism WB
gsc AB control Shield shield ISH
lft1 AB control Shield presumptive mesoderm ISH
AB + MO1-clocka standard conditions Shield presumptive mesoderm ISH
lmo2 AB control Shield angioblastic mesenchymal cell ISH
ndr2 AB control Shield whole organism ISH
AB + MO1-clocka standard conditions Shield whole organism ISH
smad2 AB + MO1-clocka standard conditions Shield whole organism RTPCR
5-9 somites whole organism RTPCR
Prim-5 whole organism RTPCR
smad3a AB + MO1-clocka standard conditions Shield whole organism RTPCR
5-9 somites whole organism RTPCR
Prim-5 whole organism RTPCR
smad4a AB + MO1-clocka standard conditions 5-9 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-smad2 WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Ab1-smad4 WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Ab1-tgfbr2 WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Ab2-smad3 WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Ab3-tgfb1 WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Ab4-smad WB AB control Shield whole organism
WB AB + MO1-clocka standard conditions Shield whole organism
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-clocka standard conditions Shield presumptive mesoderm lft1 expression decreased amount, abnormal
Shield whole organism ab-smad2 labeling decreased amount, abnormal
Shield whole organism ab2-smad3 labeling decreased amount, abnormal
Shield whole organism ab4-smad labeling decreased amount, abnormal
Shield whole organism smad2 expression decreased amount, abnormal
Shield whole organism clocka expression decreased amount, abnormal
Shield whole organism ab1-smad4 labeling decreased amount, abnormal
Shield whole organism ndr2 expression decreased amount, abnormal
Shield whole organism smad3a expression decreased amount, abnormal
5-9 somites whole organism smad4a expression decreased amount, abnormal
5-9 somites whole organism smad2 expression decreased amount, abnormal
5-9 somites whole organism smad3a expression decreased amount, abnormal
Prim-5 whole organism smad3a expression decreased amount, abnormal
Prim-5 whole organism smad2 expression decreased amount, abnormal
Prim-5 whole organism smad4a expression decreased amount, abnormal
Full text @ J. Biol. Chem.