ZFIN ID: ZDB-FIG-171101-29
Astone et al., 2015 - A GFP-Tagged Gross Deletion on Chromosome 1 Causes Malignant Peripheral Nerve Sheath Tumors and Carcinomas in Zebrafish. PLoS One   10:e0145178 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 1

Phenotype of the ia2 mutant at 24 hpf.

The homozygous mutant (C) displays a strongly delayed development and compromised phenotype, while the heterozygote (B) appears indistinguishable from the wild-type (A). The green fluorescence relative to the -8.5nkx2.2a:GFP transgene is present in heterozygous and homozygous ia2 mutant fish, being the GFP gene co-inherited with the ia2 mutant allele. This allows the early and immediate screening of the three genotypes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP unm_ia2ia2Tg/+; unm_ia2Df(Chr01:timm10,rtn4rl2a,p2rx3b,pkd2,slc27a1b)ia2a/+ standard conditions Prim-5 head IFL
Prim-5 pancreatic bud IFL
Prim-5 trunk IFL
unm_ia2ia2Tg/ia2Tg; unm_ia2Df(Chr01:timm10,rtn4rl2a,p2rx3b,pkd2,slc27a1b)ia2a/ia2a standard conditions Prim-5 head IFL
Prim-5 pancreatic bud IFL
Prim-5 trunk IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
unm_ia2ia2Tg/ia2Tg; unm_ia2Df(Chr01:timm10,rtn4rl2a,p2rx3b,pkd2,slc27a1b)ia2a/ia2a standard conditions Prim-5 embryo development arrested, abnormal
Prim-5 embryo development delayed, abnormal
Protruding-mouth whole organism decreased life span, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One