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ZFIN ID: ZDB-FIG-171101-2
Chen et al., 2015 - Nmnat1-Rbp7 Is a Conserved Fusion-Protein That Combines NAD+ Catalysis of Nmnat1 with Subcellular Localization of Rbp7. PLoS One   10:e0143825 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to Prim-5

Fig. 1

Complementary regulation of rbp7a and aldh1a2 by Nodal signaling.

[A-F] Lateral views of whole mount in situ stains of rbp7a in wild type embryos at 4hpf [A, C] 6hpf [B,D], 9hpf [E] and 10hpf [F]; [C-D] vibratome sections of 4hpf and 6hpf embryos. rbp7a signals are found in marginal cell [A-D], YSL, and in forerunner cells (arrowhead in [D-F]). [G-J] Marginal expression of aldh1a2 is strongly reduced in 6hpf MZsur [H] and MZoep [I] embryos; [J] RT-qPCR verified reduced expression levels of aldh1a2 in MZsur, MZoep as compared to wild type embryos. [K-P] Whole mount in situ hybridization for rbp7a (arrow heads) and goosecoid (gsc) (white asterisks) performed at 6 and 8 hpf in Msur+/- as control [K, N], MZsur [L, O] and MZoep [M, P] embryos. Note that expression levels of rbp7a around the YSL were similar among the gsc positive embryos and the gsc negative MZsur [B, E] and MZoep [C, F] mutants. [Q-S] rbp7a expression at 24hpf. Arrows mark rbp7a signals in the posterior midbrain that were present in MZsur [R] and MZoep embryos [S] but not in control embryo [Q]. [T] RT-qPCR results for rbp7a in of 6hpf and 24hpf embryos. Graphed is the mean and SEM from triplicate experiments. Error bars indicated the SEM. Unpaired T-test was used to test the significance (*P<0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aldh1a2 foxh1m768/+ control Shield margin ISH
foxh1m768/m768 control Shield margin ISH
Shield whole organism RTPCR
tdgf1m134/m134 control Shield margin ISH
Shield whole organism RTPCR
gsc foxh1m768/+ control Shield shield ISH
75%-epiboly axial hypoblast ISH
foxh1m768/m768 control Shield Not Detected shield ISH
75%-epiboly Not Detected axial hypoblast ISH
tdgf1m134/m134 control Shield Not Detected shield ISH
75%-epiboly Not Detected axial hypoblast ISH
rbp7a AB control Sphere marginal blastomere ISH
Shield forerunner cell group ISH
Shield yolk syncytial layer ISH
90%-epiboly forerunner cell group ISH
90%-epiboly yolk syncytial layer ISH
Bud forerunner cell group ISH
Bud yolk syncytial layer ISH
foxh1m768/+ control Shield yolk syncytial layer ISH
75%-epiboly forerunner cell group ISH
75%-epiboly yolk syncytial layer ISH
Prim-5 Not Detected midbrain posterior region ISH
foxh1m768/m768 control Shield forerunner cell group ISH
Shield whole organism RTPCR
Shield yolk syncytial layer ISH
75%-epiboly forerunner cell group ISH
75%-epiboly yolk syncytial layer ISH
Prim-5 midbrain posterior region ISH
Prim-5 whole organism RTPCR
tdgf1m134/m134 control Shield Not Detected forerunner cell group ISH
Shield whole organism RTPCR
Shield yolk syncytial layer ISH
75%-epiboly Not Detected forerunner cell group ISH
75%-epiboly yolk syncytial layer ISH
Prim-5 midbrain posterior region ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxh1m768/m768 control Shield margin aldh1a2 expression decreased amount, abnormal
Shield shield gsc expression absent, abnormal
Shield whole organism aldh1a2 expression decreased amount, abnormal
Shield whole organism rbp7a expression increased amount, abnormal
75%-epiboly axial hypoblast gsc expression absent, abnormal
Prim-5 midbrain posterior region rbp7a expression increased amount, abnormal
Prim-5 whole organism rbp7a expression increased amount, abnormal
tdgf1m134/m134 control Shield forerunner cell group rbp7a expression absent, abnormal
Shield margin aldh1a2 expression decreased amount, abnormal
Shield shield gsc expression absent, abnormal
Shield whole organism aldh1a2 expression decreased amount, abnormal
Shield whole organism rbp7a expression increased amount, abnormal
75%-epiboly axial hypoblast gsc expression absent, abnormal
75%-epiboly forerunner cell group rbp7a expression absent, abnormal
Prim-5 midbrain posterior region rbp7a expression increased amount, abnormal
Prim-5 whole organism rbp7a expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One