ZFIN ID: ZDB-FIG-171018-29
Shim et al., 2017 - Development of zebrafish medulloblastoma-like PNET model by TALEN-mediated somatic gene inactivation. Oncotarget   8:55280-55297 Full text @ Oncotarget
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Adult

Fig. 5

Immunohistochemical analysis of tumors from rb1-TALENs injected tp53 mutant zebrafish. Immunostaining was performed with lineage specific antibody with normal brain tissues (A, C, E, and G) and tumors from rb1-TALENs injected tp53 mutant zebrafish (B, D, F, and H) using anti phospho-Histone 3 (A and B), anti Zrf-1 (C and D), anti HuC/D (E and F) and anti Sox2 (G and H) antibodies. Highly mitotic tumor cells could be detected in tumor tissues from rb1-TALENs mRNA injected tp53 mutant zebrafish (B). Glial marker, Zrf-1 was scarcely stained in densely cellular region of tumor tissue (D). However, HuC/D antigen which is a postmitotic neuronal marker was strongly expressed in densely cellular region of tumor tissue (F). Neural progenitor marker, Sox2 expression was also detected in same cellular region of tumor tissue (H). Scale bars: 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC AB standard conditions Adult brain neuron
IHC tp53zdf1/zdf1 + TALEN4-rb1 standard conditions Adult brain neoplasm
Ab3-sox2 IHC AB standard conditions Adult brain
IHC tp53zdf1/zdf1 + TALEN4-rb1 standard conditions Adult brain neoplasm
zrf-1 IHC AB standard conditions Adult brain glial cell
IHC tp53zdf1/zdf1 + TALEN4-rb1 standard conditions Adult brain neoplasm
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget