header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171018-28
Shim et al., 2017 - Development of zebrafish medulloblastoma-like PNET model by TALEN-mediated somatic gene inactivation. Oncotarget   8:55280-55297 Full text @ Oncotarget
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Adult

Fig. 4

Histopathology of tumors from rb1-TALENs injected tp53 mutant zebrafish. (A) Sagittal section image of H & E staining of wild type zebrafish at 5 month post fertilization. Black arrows and arrow heads indicate forebrain and optic tectum, respectively. Cerebellum and cerebellar granule cells are indicated by white arrows and arrow heads, respectively. (B) High magnitude image of A. Normal cerebellar granule cells have a unique feature that is characterized with small rounded nuclei. (CH) Sagittal section images of H & E staining of tumors from rb1-TALENs injected tp53 mutant zebrafish. (C) Tumors were mainly arising in cerebellum, medulla, and brainstem. (D) High magnitude image of C. Highly cellular tumor cells with wedged nuclei were observed. Rosette like structures was marked by white arrows. (E) Tumors were mainly arising in cerebellum encompassed a majority of medulla, hypothalamus, and brainstem. The forth ventricle which were surrounded with tumor cells were marked by black arrows. (F) High magnitude image of E. Infiltration of tumor cells was indicated by black arrowheads in dorsal brainstem. (H) High magnitude image of G. Homer-Wright rosettes which are seen in PNETs or medulloblastomas were observed distinctly (white arrows). Anterior is left of all images. Scale bars: 500 μm (A, C, E and G), and 50 μm (B, D, F and H).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tp53zdf1/zdf1 + TALEN4-rb1 standard conditions Adult brain medulloblastoma increased amount, abnormal
Adult brainstem neoplastic, abnormal
Adult brainstem neoplasm increased amount, abnormal
Adult cerebellum neoplastic, abnormal
Adult cerebellum neoplasm increased amount, abnormal
Adult medulla oblongata neoplastic, abnormal
Adult medulla oblongata neoplasm increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget