header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171018-26
Shim et al., 2017 - Development of zebrafish medulloblastoma-like PNET model by TALEN-mediated somatic gene inactivation. Oncotarget   8:55280-55297 Full text @ Oncotarget
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Adult

Fig. 2

Immunohistochemical analysis of tumors from cdkn2a/b TALENs mRNA injected tp53 mutant zebrafish. (A) (A') Immunostaining with anti- phospho-Histone 3 antibidy. The tumor tissue from cdkn2a/b TALENs mRNA injected tp53 mutant zebrafish is highly mitotic. The phospho-Histone 3 positive mitotic cells were more evident in the outer region of tumor tissue (red arrowhead in A). (B) (B') Neuronal signature that was visualized by immunostaining with anti-HuC/D antibody was scarcely detected in densely cellular region of tumor tissue. (C) (C') Immunostaining with zrf-1 which detect zebrafish GFAP protein. The tumor tissue induced with cdkn2a/b TALENs mRNA also did not show any glial characteristics. Normal staining of anti-HuC/D and zrf-1 antibody could be observed in retinal layers near to tumor tissues (Inset box in B' and C'). (D) (D') Epithelioid MPNST marker, S100β, is strongly expressed in tumor tissue. (E) (E') Mesenchymal marker, Vimentin, is also positively stained in tumor tissue. Scale bars: 1 mm (A) and 100 μm (A').

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC tp53zdf1/zdf1 + TALEN1-cdkn2a/b standard conditions Adult retina
IHC tp53zdf1/zdf1 + TALEN2-cdkn2a/b standard conditions Adult retina
Ab4-s100b IHC tp53zdf1/zdf1 + TALEN1-cdkn2a/b standard conditions Adult eye neoplasm
IHC tp53zdf1/zdf1 + TALEN2-cdkn2a/b standard conditions Adult eye neoplasm
Ab7-vim IHC tp53zdf1/zdf1 + TALEN1-cdkn2a/b standard conditions Adult eye neoplasm
IHC tp53zdf1/zdf1 + TALEN2-cdkn2a/b standard conditions Adult eye neoplasm
zrf-1 IHC tp53zdf1/zdf1 + TALEN1-cdkn2a/b standard conditions Adult retina
IHC tp53zdf1/zdf1 + TALEN2-cdkn2a/b standard conditions Adult retina
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget