ZFIN ID: ZDB-FIG-171009-18
Raices et al., 2017 - Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex. Developmental Cell   41:540-554.e7 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to Long-pec

Fig. 2

Nup210 Depletion Does Not Affect Early Muscle Development

(A) Whole-mount in situ hybridization against the muscle markers Pax3, Myf5, and MyoD were performed at different times of development in zebrafish embryos injected with control or Nup210 morpholinos.

(B) Slow muscle was stained with F59 at different times of development in control and Nup210-depleted animals and analyzed by confocal microscopy.

(C) Control or Nup210 morphants were incubated with cyclopamine during development to inhibit the Sonic Hedgehog-dependent formation of early embryonic slow muscle. Addition of new muscle fibers during muscle growth was analyzed by F59 staining at 48 hpf.

Morpholino depletions were performed with n ≥ 50 embryos. Ten to 20 embryos were examined by immunofluorescence and quantified in n ≥ 3 independent experiments. Scale bars, 50 μm. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myf5 TL control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
TL + MO1-nup210 control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
myod1 TL control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
TL + MO1-nup210 control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
pax3a TL control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
TL + MO1-nup210 control 5-9 somites muscle cell ISH
10-13 somites muscle cell ISH
14-19 somites muscle cell ISH
Prim-5 muscle cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC TL control 14-19 somites slow muscle cell
IHC Prim-5 slow muscle cell
IHC Long-pec slow muscle cell
IHC TL chemical treatment by environment: Cyclopamine Long-pec slow muscle cell
IHC TL + MO1-nup210 control 14-19 somites slow muscle cell
IHC Prim-5 slow muscle cell
IHC Long-pec slow muscle cell
IHC TL + MO1-nup210 chemical treatment by environment: Cyclopamine Long-pec slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO1-nup210 control 5-9 somites skeletal muscle fiber development process quality, normal
10-13 somites skeletal muscle fiber development process quality, normal
14-19 somites skeletal muscle fiber development process quality, normal
Prim-5 skeletal muscle fiber development process quality, normal
Long-pec muscle cell detached from myoseptum, abnormal
Long-pec muscle cell structure, abnormal
Long-pec skeletal muscle fiber development decreased process quality, abnormal
TL + MO1-nup210 chemical treatment by environment: Cyclopamine Long-pec skeletal muscle fiber development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 41, Raices, M., Bukata, L., Sakuma, S., Borlido, J., Hernandez, L.S., Hart, D.O., D'Angelo, M.A., Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex, 540-554.e7, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell