FIGURE

Fig. S4

ID

ZDB-FIG-170914-59

Publication

Askary et al., 2017 - Genome-wide analysis of facial skeletal regionalization in zebrafish

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Fig. S4

Analysis of emx2; edn1 compound mutants. (A) Alcian blue and Alizarin red staining of control, emx2, edn1, and emx2; edn1 double mutant embryos at 5 dpf. In edn1 mutants, the opercle bone is variably expanded / normal (closed arrowhead) or reduced / absent (open arrowhead). In emx2 and emx2; edn1 mutants, the opercle is almost always reduced or absent. In addition, loss of emx2 fails to rescue the reduced ventral cartilage in edn1 mutants. Scale bar = 100 μm. (B) Quantification of the percentage of opercle bone defects in single and double mutants. The number of sides examined is listed above each bar. The decrease in the proportion of larvae with expanded / normal opercle bone in emx2^-/-; edn1^-/- mutants compared with emx2+/+; edn1^-/- was significant at p < 0.0001 (two-tailed Chi-square).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	edn1^{tf216b/tf216b} emx2^el586/el586 edn1^{tf216b/tf216b}; emx2^el586/el586
Observed In:	opercle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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