ZFIN ID: ZDB-FIG-170914-4
Quach et al., 2015 - A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish. G3 (Bethesda)   5(6):1283-99 Full text @ G3 (Bethesda)
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 7

Protein trap-induced mutant phenotype affecting the dhx37 gene. (A–D) Bright field images of dhx37ws0977Tg/+ sibling (A), homozygous dhx37ws0977Tg/ws0977Tg mutant (B), dhx37 ATG morpholino-injected (C), or full-length dhx37 RNA-injected homozygous mutant embryo (D). (E) The Ds insertion in chr8:4765207-4765215 is linked to the mutant phenotype. Black arrows indicate the position of primers used for genotyping PCRs. (F–H) RT-PCR results from homozygous dhx37ws0977Tg/ws0977Tg embryos show that the bcl2 splice acceptor sequence trapped endogenous upstream splice donor sites in the dhx37 gene, producing multiple fusion transcripts (F, G), leading to the disruption of wild-type transcripts (H). (G) The main fusion products, 1 and 2, are out of frame with respect to the reading sequence. Black arrows in the schematic in (F) indicate the positions of primers used for RT-PCR. No evidence of wild-type transcripts was found, and only mutant fusion transcripts were detected. The fusion transcripts always have a 71-bp linker sequence (SA) originated from bcl2 splice acceptor site left between upstream exons and the mCherry reporter sequence. The major fusion transcripts 1 and 2 are out of frame for mCherry coding sequences (G).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-dhx37 standard conditions Day 5 brain anterior region decreased size, abnormal
Day 5 pericardium edematous, abnormal
Day 5 whole organism anterior-posterior axis decreased length, abnormal
dhx37ws09771Tg/ws09771Tg standard conditions Day 5 brain anterior region decreased size, abnormal
Day 5 pericardium edematous, abnormal
Day 5 whole organism anterior-posterior axis decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal G3 (Bethesda) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ G3 (Bethesda)