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ZFIN ID: ZDB-FIG-170912-22
Lamont et al., 2016 - The LIM-homeodomain transcription factor Islet2a promotes angioblast migration. Developmental Biology   414:181-92 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 3

Loss of Isl2a interferes with angioblast migration and fli1a expression. (A) qPCR expression of angioblast genes. (B-I) Expression of etsrp/etv2 in wild type (B,D,F,H), isl2MO (C,E) or isl2aca21 mutants (G,I) as seen in dorsal or posterior views. Red bracket denotes expression at posterior of embryo.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etsrp TL control 10-13 somites to 14-19 somites posterior lateral mesoderm angioblastic mesenchymal cell ISH
Prim-5 whole organism RTPCR
TL + MO4-isl2a + MO5-isl2a control 10-13 somites to 14-19 somites posterior lateral mesoderm angioblastic mesenchymal cell ISH
Prim-5 whole organism RTPCR
isl2aca21/ca21 control 10-13 somites to 14-19 somites posterior lateral mesoderm angioblastic mesenchymal cell ISH
fli1 TL control Prim-5 whole organism RTPCR
TL + MO4-isl2a + MO5-isl2a control Prim-5 whole organism RTPCR
gata1a TL control Prim-5 whole organism RTPCR
TL + MO4-isl2a + MO5-isl2a control Prim-5 whole organism RTPCR
vegfc TL control Prim-5 whole organism RTPCR
TL + MO4-isl2a + MO5-isl2a control Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO4-isl2a + MO5-isl2a control 10-13 somites - 14-19 somites angioblastic mesenchymal cell decreased amount, abnormal
10-13 somites - 14-19 somites angioblastic mesenchymal cell irregular spatial pattern, abnormal
10-13 somites - 14-19 somites angioblastic mesenchymal cell mislocalised, abnormal
10-13 somites - 14-19 somites posterior lateral mesoderm angioblastic mesenchymal cell etsrp expression spatial pattern, abnormal
Prim-5 whole organism fli1 expression decreased amount, abnormal
isl2aca21/ca21 control 10-13 somites - 14-19 somites angioblastic mesenchymal cell decreased amount, abnormal
10-13 somites - 14-19 somites angioblastic mesenchymal cell irregular spatial pattern, abnormal
10-13 somites - 14-19 somites angioblastic mesenchymal cell mislocalised, abnormal
10-13 somites - 14-19 somites posterior lateral mesoderm angioblastic mesenchymal cell etsrp expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 414, Lamont, R.E., Wu, C.Y., Ryu, J.R., Vu, W., Davari, P., Sobering, R.E., Kennedy, R.M., Munsie, N.M., Childs, S.J., The LIM-homeodomain transcription factor Islet2a promotes angioblast migration, 181-92, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.