ZFIN ID: ZDB-FIG-170907-59
Fukuda et al., 2017 - Proteolysis regulates cardiomyocyte maturation and tissue integration. Nature communications   8:14495 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 2

asb2b mutant cardiomyocytes exhibit structural defects.

(a) Three-dimensional images of 50 hpf Tg(myl7:LIFEACT-GFP) WT and asb2b mutant hearts reveal differences in myofilament thickness and organization. Magnified views of myofilaments in white boxes are shown on the right. Myofilament thickness was measured in 50 hpf WT and asb2b mutant hearts (n=29 myofilaments from 5 hearts). (b) Three-dimensional images of 50 hpf Tg(myl7:LIFEACT-GFP);Tg(myl7:MKATE-CAAX) WT and asb2b mutant atria. The myofilaments in WT are organized across cell borders (arrows), whereas asb2b mutant hearts show disorganized myofilaments between adjacent cardiomyocytes (arrows). (c) Three-dimensional images of 50 hpf Tg(myl7:actn3b-EGFP) WT and asb2b mutant atria. Atrial cardiomyocytes in WT exhibit an organized z-band pattern across cell–cell borders (arrows), whereas those in asb2b mutants exhibit a disorganized z-band pattern (arrows). (d) Three-dimensional images of 50 hpf Tg(myl7:ras-GFP) WT and asb2b mutant hearts. Magnified views of myofilaments in white dotted boxes are shown on the right. Cardiomyocytes are outlined to define shape. (e) Single-plane images of 50 hpf Tg(myl7:EGFP-Podocalyxin);Tg(myl7:MKATE-CAAX) WT and asb2b mutant atria. In WT, EGFP-Podocalyxin is localized on the abluminal (apical) side of cardiomyocytes, whereas in asb2b mutants, it appears to be localized on both the abluminal and luminal (basal) sides (arrows). Number of cardiomyocytes exhibiting EGFP-Podocalyxin localization on the basal side in WT and asb2b mutant hearts (n=5 hearts, with averages taken from 20 cells per heart). *P<0.05 and **P<0.01 by one-way analysis of variance (ANOVA) followed by Tukey’s honest significant difference test. Error bars, s.e.m. Scale bars, 20 μm. At, atrium; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP asb2bbns33/bns33 ; sd10Tg ; sd11Tg standard conditions Long-pec cardiac muscle cell Z disc IFL
bns103Tg ; sd11Tg standard conditions Long-pec cardiac muscle cell apical side IFL
s883Tg standard conditions Long-pec cardiac muscle cell plasma membrane IFL
s883Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell plasma membrane IFL
sd10Tg; sd11Tg standard conditions Long-pec cardiac muscle cell Z disc IFL
sd11Tg ; bns103Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell basal side IFL
GFP s974Tg standard conditions Long-pec cardiac muscle cell myofilament IFL
s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament IFL
sd11Tg ; s974Tg standard conditions Long-pec cardiac muscle cell myofilament IFL
sd11Tg ; s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament IFL
MKATE2 asb2bbns33/bns33 ; sd10Tg ; sd11Tg standard conditions Long-pec cardiac muscle cell plasma membrane IFL
bns103Tg ; sd11Tg standard conditions Long-pec cardiac muscle cell plasma membrane IFL
sd10Tg; sd11Tg standard conditions Long-pec cardiac muscle cell plasma membrane IFL
sd11Tg ; bns103Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell plasma membrane IFL
sd11Tg ; s974Tg standard conditions Long-pec cardiac muscle cell plasma membrane IFL
sd11Tg ; s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell plasma membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
asb2bbns33/bns33 ; sd10Tg ; sd11Tg standard conditions Long-pec cardiac muscle cell Z disc disorganized, abnormal
s883Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell shape, abnormal
Long-pec cardiac muscle cell plasma membrane has extra parts of type cardiac muscle cell cell projection, abnormal
s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament decreased thickness, abnormal
s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament disorganized, abnormal
s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament misaligned with cardiac muscle cell myofilament, abnormal
sd11Tg ; bns103Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell basal side EGFP expression mislocalised, abnormal
sd11Tg ; s974Tg ; asb2bbns33/bns33 standard conditions Long-pec cardiac muscle cell myofilament disorganized, abnormal
Long-pec cardiac muscle cell myofilament misaligned with cardiac muscle cell myofilament, abnormal
Long-pec cardiac muscle cell myofilament separated from cardiac muscle cell myofilament, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.