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ZIRC
ZFIN ID: ZDB-FIG-170907-37
Gerri et al., 2017 - Hif-1α regulates macrophage-endothelial interactions during blood vessel development in zebrafish. Nature communications   8:15492 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin

Fig. 8

Most macrophages closely associated with unstable vessels are tnfa:EGFPF+.

(a) Schematic representation of the experiment shown in bd. (b) Quantification at 66 hpf of tnfa:EGFPF+ and tnfa:EGFPF macrophages in normoxia and after DMOG treatment for 18 h starting at 48 hpf. (c) Maximal intensity projections of time-lapse confocal images of Tg(mpeg:mCherry);Tg(tnfa:EGFPF);Tg(kdrl:mCherry) WT embryo treated with DMOG starting at 48 hpf; lateral views. Yellow arrowhead points to an unstable vessel, white arrowheads point to recruited macrophages. (d) Quantification at 66 hpf of tnfa:EGFPF+ and tnfa:EGFPF macrophages associated with 15 unstable vessels after DMOG treatment starting at 48 hpf. 38 macrophages were observed, 23 tnfa:EGFPF+ and 15 tnfa:EGFPF. Bars represent mean±s.e.m., n=15 embryos from three different clutches, (*P<0.05; NS, no significant changes observed; t-test). Scale bar, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ump2Tg ; s896Tg ; ump5Tg chemical treatment by environment: dimethyloxalylglycine Long-pec to Pec-fin macrophage IFL
mCherry ump2Tg ; s896Tg ; ump5Tg chemical treatment by environment: dimethyloxalylglycine Long-pec to Pec-fin dorsal longitudinal anastomotic vessel IFL
Long-pec to Pec-fin endothelial cell IFL
Long-pec to Pec-fin macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.