ZFIN ID: ZDB-FIG-170907-33
Gerri et al., 2017 - Hif-1α regulates macrophage-endothelial interactions during blood vessel development in zebrafish. Nature communications   8:15492 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4

Macrophage ablation leads to the absence of DLAV plexus formation as well as hypoxia-induced vascular disconnections.

(a) Schematic representation of the experiment shown in bd. (b,c) Maximal intensity projections of time-lapse confocal images of control Tg(kdrl:EGFP);Tg(mpeg:NTR-mCherry) and macrophage-ablated embryos in normoxia starting at 30 hpf. Arrowheads point to a macrophage co-localizing with EC sprouts. (d) Quantification of embryos showing normal (grey) or abnormal/absent (red) DLAV plexus formation in 54 hpf control and macrophage-ablated embryos. (e) Schematic representation of the experiment shown in fm. (fk) Maximal intensity projections of confocal z-stacks of 54 hpf control Tg(kdrl:EGFP);Tg(mpeg:NTR-mCherry) and macrophage-ablated embryos in normoxia, after hypoxia chamber or DMOG treatment for 6 h starting at 48 hpf. All images represent lateral views. Arrowheads point to macrophages in proximity to blood vessels, and arrows to vessel ruptures. (l) Quantification of blood vessel ruptures in a 10 somite-long trunk area in 54 hpf control and macrophage-ablated embryos in normoxia, after hypoxia chamber or DMOG treatment for 6 h starting at 48 hpf. (m) Quantification of macrophages in proximity to blood vessels in a 10 somite-long trunk area in 54 hpf control and macrophage-ablated embryos in normoxia, after hypoxia chamber or DMOG treatment for 6 h starting at 48 hpf. n=10 embryos for each condition. (n) Quantification of embryos showing normal (grey) or abnormal/absent (red) DLAV plexus formation in 54 hpf control and macrophage-ablated hif-1α−/−embryos. (o) Quantification of blood vessel ruptures in a 10 somite-long trunk area in 54 hpf control and macrophage-ablated hif-1α−/−embryos in normoxia, after hypoxia chamber or DMOG treatment for 6 h starting at 48 hpf. Bars represent mean±s.e.m., n=10 embryos from three different clutches, (***P<0.001; ****P<0.0001; NS, no significant changes observed; t-test). Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP c264Tg; gl24Tg; s843Tg control Prim-15 to High-pec dorsal longitudinal anastomotic vessel IFL
Prim-15 to High-pec intersegmental vessel IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
c264Tg; gl24Tg; s843Tg chemical treatment by environment: dimethyloxalylglycine Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole Prim-15 to High-pec dorsal longitudinal anastomotic vessel IFL
Prim-15 to High-pec intersegmental vessel IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole, chemical treatment by environment: dimethyloxalylglycine Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
c264Tg; gl24Tg; s843Tg hypoxia Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
c264Tg; gl24Tg; s843Tg hypoxia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec trunk vasculature IFL
mCherry c264Tg; gl24Tg; s843Tg control Prim-15 to High-pec macrophage IFL
Long-pec macrophage IFL
c264Tg; gl24Tg; s843Tg chemical treatment by environment: dimethyloxalylglycine Long-pec macrophage IFL
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole Prim-15 to High-pec macrophage IFL
Long-pec Not Detected macrophage IFL
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole, chemical treatment by environment: dimethyloxalylglycine Long-pec Not Detected macrophage IFL
c264Tg; gl24Tg; s843Tg hypoxia Long-pec macrophage IFL
c264Tg; gl24Tg; s843Tg hypoxia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Long-pec Not Detected macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole Prim-15 - High-pec dorsal longitudinal anastomotic vessel anatomical region has fewer parts of type macrophage, abnormal
Prim-15 - High-pec dorsal longitudinal anastomotic vessel blood vessel development decreased occurrence, abnormal
Prim-15 - High-pec dorsal longitudinal anastomotic vessel malformed, abnormal
Prim-15 - High-pec macrophage absent, abnormal
Long-pec dorsal longitudinal anastomotic vessel anatomical region has fewer parts of type macrophage, abnormal
Long-pec macrophage absent, abnormal
Long-pec macrophage mCherry expression absent, abnormal
c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole, chemical treatment by environment: dimethyloxalylglycine Long-pec dorsal longitudinal anastomotic vessel anatomical region has fewer parts of type macrophage, abnormal
Long-pec intersegmental vessel broken, abnormal
Long-pec macrophage absent, abnormal
Long-pec macrophage mCherry expression absent, abnormal
c264Tg; gl24Tg; s843Tg hypoxia, chemical ablation: macrophage, chemical treatment by environment: metronidazole Long-pec dorsal longitudinal anastomotic vessel anatomical region has fewer parts of type macrophage, abnormal
Long-pec intersegmental vessel broken, abnormal
Long-pec macrophage absent, abnormal
Long-pec macrophage mCherry expression absent, abnormal
hif1abbns90/bns90; hif1aabns89/bns89; c264Tg; gl24Tg; s843Tg control Long-pec dorsal longitudinal anastomotic vessel blood vessel development decreased occurrence, abnormal
Long-pec dorsal longitudinal anastomotic vessel malformed, abnormal
Long-pec intersegmental vessel broken, abnormal
hif1abbns90/bns90; hif1aabns89/bns89; c264Tg; gl24Tg; s843Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole Long-pec dorsal longitudinal anastomotic vessel blood vessel development decreased occurrence, abnormal
Long-pec dorsal longitudinal anastomotic vessel malformed, abnormal
Long-pec intersegmental vessel broken, abnormal
Acknowledgments:
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