FIGURE

Fig. 2

ID

ZDB-FIG-170907-12

Publication

Pfefferli et al., 2017 - The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

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Fig. 2

ctgfa^careg:EGFP does not reproduce endogenous ctgfa expression in peri-injury tissues.

(a–h) In situ hybridization using ctgfa and enhanced green fluorescent protein (egfp) probes on sections of hearts at 14 d.p.ci. and fins at 3 d.p.a. of ctgfa^careg:EGFP transgenic fish shows distinct expression patterns. (a,b) ctgfa mRNA is not detected in the myocardium, but in a subset of non-myocytes in the post-infarcted tissue. (c,d) egfp mRNA is expressed in the outer wall of the myocardium and at the peri-injury zone (yellow arrowheads). (e,f) ctgfa mRNA is present in a few bone-associated cells of the fin, while (g,h) egfp mRNA is detected in the blastema. N=6. (i–m) careg:EGFP and careg:dmKO2 double transgenic fish display an overlapping expression pattern in the regenerating heart at 7 d.p.ci. and the fin at 1 d.p.a. N=4.

Expression Data

Genes:	ccn2a EGFP mKOFP2
Fish:	zf620Tg fri1Tg; zf620Tg
Conditions:	amputation: caudal fin cryoablation: cardiac ventricle
Anatomical Terms:	blastema mesenchymal cell cardiac ventricle cardiac muscle cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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