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ZFIN ID: ZDB-FIG-170829-5
Abrial et al., 2017 - TGF-β Signaling Is Necessary and Sufficient for Pharyngeal Arch Artery Angioblast Formation. Cell Reports   20:973-983 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 6

Premature TGF-β Signaling Induces Precocious Differentiation of tie1+ PAA Angioblasts

(A) Experimental design for heat-shock-mediated (HS+) induction of TGF-β signaling in Tg(hsp70:caAlk5) embryos at 14 hpf (10 somites stage) followed by analysis of etsrp+ and tie1+ PAA angioblasts at 24 or 28 hr post-fertilization (hpf), respectively. Transgenic embryos not exposed to heat shock (HS, Tg+) and heat shocked wild-type embryos (HS+, Tg–) were used as controls.

(B–G) Left lateral images of 24-hpf (B–D) or 28-hpf (E–G) control (B, C, E, and F) and experimental (D and G) embryos. Two large etsrp+ or tie1+ PAA angioblast clusters are visible in (D) and (G), respectively, whereas the majority of control embryos (B, C, E, and F) lacked PAA angioblasts at this stage. Scale bars, 200 μm.

(H and I) Bar graphs showing the average number of etsrp+ or tie1+ angioblast clusters ±SD within each experimental group (p < 0.05; ∗∗p < 0.01). n = 40–50 embryos per group with a total of three biological replicates.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etsrp WT control Prim-5 angioblastic mesenchymal cell ISH
fb6Tg control Prim-5 angioblastic mesenchymal cell ISH
fb6Tg heat shock Prim-5 pharyngeal arch 3 angioblastic mesenchymal cell ISH
Prim-5 pharyngeal arch 4 angioblastic mesenchymal cell ISH
tie1 WT control Prim-5 angioblastic mesenchymal cell ISH
fb6Tg control Prim-5 angioblastic mesenchymal cell ISH
fb6Tg heat shock Prim-5 pharyngeal arch 3 angioblastic mesenchymal cell ISH
Prim-5 pharyngeal arch 4 angioblastic mesenchymal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fb6Tg heat shock Prim-5 pharyngeal arch angioblastic mesenchymal cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.