ZFIN ID: ZDB-FIG-170807-24
Passoni et al., 2017 - Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes. Disease models & mechanisms   10(7):847-857 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 4

SINV does not induce BBB leakage. In vivo assay for BBB permeability. Uninfected controls, IHNV- and SINV-infected fliG/U:RFP larvae were injected IV at 1 and 2 dpi with 10 kDa FITC-dextran. One hour later, live confocal imaging of the brain was performed, to compare FITC intensity within the CBV and BP. (Injections at 2 dpi were performed only on uninfected controls and SINV-infected larvae, because of early death of IHNV-infected larvae.) (A,B) Representative images of IHNV-infected (A) and SINV-infected (B) larvae; single confocal plane at same depth. Scale bars: 100 μm. (C) Ratios of FITC fluorescence intensities measured in BP and CBV. n=2-5 larvae per group; two separate focal planes per larva.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
RFP nkuasrfp1aTg; ubs4Tg viral treatment by injection: Infectious hematopoietic necrosis virus Day 4 endothelial cell IFL
nkuasrfp1aTg; ubs4Tg viral treatment by injection: Sindbis virus Day 4 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nkuasrfp1aTg; ubs4Tg viral treatment by injection: Infectious hematopoietic necrosis virus Day 4 maintenance of blood-brain barrier discontinuous, abnormal
Acknowledgments:
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