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ZFIN ID: ZDB-FIG-170517-8
Jiang et al., 2017 - Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 10

emx2 regulates hair bundle polarity in neuromasts.

(A) Surface view of an A-P and D-V oriented neuromast and a sagittal view of the cellular architecture of a neuromast in the lateral line of zebrafish. (B) HCs with opposite orientation are in a 1:1 ratio. (C–C'') An example and (C''') quantification showing only HCs (parvalbumin-positive, blue) with hair bundles (phalloidin, red) oriented toward the posterior (C, asterisks) or ventral direction are positive for anti-Emx2 staining (green) in A-P and D-V neuromasts, respectively (55 neuromasts from 26 larvae, ***p<0.001; Figure 10—source data 1). (D–D'') An example and (D”') quantification of HCs in emx2 knockouts showing all HCs are emx2-negative (D') and pointing towards the same direction (36 neuromasts, 18 larvae, ***p<0.001; Figure 10—source data 1). (E–E''') All hair bundles (visualized using m6b:βactin-GFP, red) are Emx2-positive (E',E'', green) and pointing toward the posterior in the A-P oriented neuromasts of gof mutants. (E''') Quantification of hair bundle polarity in 43 A-P and D-V neuromasts from 23 larvae (Figure 10—source data 1). ***p<0.001. (F–J) In response to an anterior-to-posterior stimulus (F') or posterior-to-anterior stimulus (G') a similar percentage of wildtype HCs show mechanically-evoked increase in calcium levels (J; Figure 10—source data 2). Emx2 gof HCs only respond to an anterior-to-posterior stimulus (H') and are inhibited by a posterior-to-anterior stimulus (I'). The heatmap indicates the change in calcium levels during the first half of the 2s-step stimulus (2 larvae and 4 neuromasts in control, 2 larvae and 7 neuromasts in emx2 gof). Error bar represents SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP idc4Tg ; vo8Tg standard conditions Day 4 neuromast hair cell stereocilium bundle IHC
Day 4 posterior lateral line neuromast neuromast hair cell IHC
emx2 Ab1-emx2 WT standard conditions Day 4 posterior lateral line neuromast neuromast hair cell IHC
Ab1-emx2 emx2idc5/idc5 standard conditions Day 4 Not Detected posterior lateral line neuromast neuromast hair cell IHC
GCaMP idc1Tg standard conditions Day 4 posterior lateral line neuromast neuromast hair cell IFL
idc1Tg ; idc4Tg standard conditions Day 4 posterior lateral line neuromast neuromast hair cell IFL
mCherry idc4Tg ; vo8Tg standard conditions Day 4 posterior lateral line neuromast neuromast hair cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC WT standard conditions Day 4 posterior lateral line neuromast neuromast hair cell
IHC emx2idc5/idc5 standard conditions Day 4 posterior lateral line neuromast neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
emx2idc5/idc5 standard conditions Day 4 neuromast hair cell stereocilium bundle orientation neuromast, abnormal
Day 4 neuromast stereocilium bundle bilateral symmetry, abnormal
Day 4 neuromast stereocilium bundle unidirectional, abnormal
Day 4 posterior lateral line neuromast hair cell development decreased process quality, abnormal
Day 4 posterior lateral line neuromast neuromast hair cell emx2 expression absent, abnormal
Day 4 posterior lateral line posterior lateral line neuromast development decreased process quality, abnormal
idc1Tg ; idc4Tg standard conditions Day 4 posterior lateral line neuromast decreased functionality, abnormal
Day 4 posterior lateral line neuromast detection of mechanical stimulus involved in sensory perception decreased process quality, abnormal
Day 4 posterior lateral line neuromast neuromast hair cell GCaMP expression spatial pattern, abnormal
idc4Tg ; vo8Tg standard conditions Day 4 neuromast hair cell stereocilium bundle orientation neuromast, abnormal
Day 4 neuromast stereocilium bundle bilateral symmetry, abnormal
Day 4 neuromast stereocilium bundle unidirectional, abnormal
Day 4 posterior lateral line neuromast hair cell development decreased process quality, abnormal
Day 4 posterior lateral line posterior lateral line neuromast development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife