ZFIN ID: ZDB-FIG-170501-1
Seberg et al., 2017 - TFAP2 paralogs regulate melanocyte differentiation in parallel with MITF. PLoS Genetics   13:e1006636 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 1

Melanocyte differentiation genes are sensitive to the loss of TFAP2A.

(A, B) Lateral images of tfap2a+/- and tfap2a-/- mutant zebrafish at 36 hpf. tfap2a-/- mutants have fewer melanocytes and delayed pigmentation relative to their wildtype or tfap2a+/- siblings. (C) Fold changes calculated from microarray expression levels for 20 zebrafish melanocyte genes. Top: 11 genes with expression levels between 0.2–0.55-fold in tfap2a-/- mutants compared to WT siblings. Bottom: 9 genes with expression levels reduced no further than the number of melanocytes in tfap2a-/- mutants. (D) qRT-PCR validation of zebrafish microarray results for 13 melanocyte genes (Mann-Whitney U, *p<0.05, **p<0.01). (E) qRT-PCR validation of microarray results for 12 melanocyte genes in mouse melan-a cells transfected with control siRNA or each of two siRNAs targeting Tfap2a (siA, si5). Significance evaluated only when both siA and si5 altered gene expression in the same direction relative to siCtrl (one-way ANOVA with Bonferroni correction, ****p<0.0001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
mc1r tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
oca2 tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
pmela tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
slc45a2 tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
tyr tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
tyrp1b tfap2ats213/ts213 standard conditions Prim-25 trunk RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2ats213/ts213 standard conditions Prim-25 melanocyte decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 pigmentation delayed, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk tyr expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk mc1r expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk pmela expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk tyrp1b expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk slc45a2 expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk dct expression decreased amount, abnormal
tfap2ats213/ts213 standard conditions Prim-25 trunk oca2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.