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ZFIN ID: ZDB-FIG-170315-8
Zhang et al., 2015 - G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition. Cell Research   25:1093-107 Full text @ Cell Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-25 to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to Days 45-89

Fig. 2

Gpr183 plays an indispensable role during definitive hematopoiesis in zebrafish. (A) Generation of gpr183 mutant using the CRISPR/Cas9 technique. PAM sequence is 'TGG' (in blue); alignment of WT and mutated sequences is listed below. (B, C) Reduced expression of cmyb (or runx1) and rag1 in gpr183 mutant embryos (B) and clotrimazole-treated embryos (C). Red arrowheads indicate cmyb or runx1 expression in AGM, and white circles denote rag1 expression in the thymus. (D) The number of HE in Tg(gpr183−/−;flk1:mCherry/runx1:en-GFP) embryos is attenuated significantly compared with that in control embryos. White arrowheads denote HE in the AGM region. (E) Dot-plot quantification analysis of HE in sibling control and gpr183 mutant. Error bar represents SD. ****P < 0.0001. (F) Hematoxylin-eosin staining of head kidney section showing hematopoietic defect in 12-week-old gpr183 heterozygous mutant fish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP gpr183aioz9/ioz9 ; s896Tg ; ioz1Tg control Prim-25 angioblastic mesenchymal cell IFL
Prim-25 ventral wall of dorsal aorta IFL
ioz1Tg; s896Tg control Prim-25 angioblastic mesenchymal cell IFL
Prim-25 ventral wall of dorsal aorta IFL
mCherry gpr183aioz9/ioz9 ; s896Tg ; ioz1Tg control Prim-25 angioblastic mesenchymal cell IFL
Prim-25 ventral wall of dorsal aorta IFL
ioz1Tg; s896Tg control Prim-25 angioblastic mesenchymal cell IFL
Prim-25 ventral wall of dorsal aorta IFL
myb AB/TU control Prim-25 ventral wall of dorsal aorta ISH
gpr183aioz9/ioz9(AB/TU) control Prim-25 ventral wall of dorsal aorta ISH
rag1 AB/TU control Day 5 thymus ISH
TU control Day 5 thymus ISH
TU chemical treatment: clotrimazole Day 5 thymus ISH
gpr183aioz9/ioz9(AB/TU) control Day 5 thymus ISH
runx1 TU control Prim-25 ventral wall of dorsal aorta ISH
TU chemical treatment: clotrimazole Prim-25 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment: clotrimazole Prim-25 ventral wall of dorsal aorta runx1 expression decreased amount, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
gpr183aioz9/ioz9(AB/TU) control Prim-25 ventral wall of dorsal aorta myb expression decreased amount, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
Days 45-89 definitive hemopoiesis decreased occurrence, abnormal
Days 45-89 head kidney decreased size, abnormal
Days 45-89 head kidney cell decreased amount, abnormal
gpr183aioz9/ioz9 ; s896Tg ; ioz1Tg control Prim-25 angioblastic mesenchymal cell GFP expression decreased amount, abnormal
Prim-25 angioblastic mesenchymal cell mCherry expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta mCherry expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta GFP expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta angioblastic mesenchymal cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Res.