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ZFIN ID: ZDB-FIG-170123-40
Hou et al., 2017 - The Sec domain protein Scfd1 facilitates trafficking of ECM components during chondrogenesis. Developmental Biology   421(1):8-15 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 3

Protein trafficking is disrupted in scfd1 mutants. (A–D) Chondrocytes in scfd1 mutant embryo express col2a1a normally at 72 hpf, with reduced expression by 96 hpf. A to D, ventral views with anterior to the top. (E-H) Toluidine blue staining of transverse sections of the jaw at 80 hpf. Nuclei are stained blue, whereas ECM is stained purple. (I–P) Immunostaining of Collagen II and WGA in the palatoquadrate of 80 hpf wild type and scfd1 mutant embryos. Arrowheads in L and P indicate aberrant intracellular ECM protein localization in a scfd1 mutant. (Q–T) TEM images of 80 hpf wild type and scfd1 mutant chondrocytes. Black arrows indicate well-stacked ER in wild type cell (R) or distended ER membranes in scfd1 mutant cells (T). Orange arrowheads point to collagen fibril deposition in the extracellular space. Scale bars: 25 µm (F, H, J, L, N and P); 2 µm (Q–T). For A to P, at least 15 embryos for each genotype were analyzed and representative samples are shown. For Q to T, 6 embryos for each genotype were analyzed and representative samples are shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT standard conditions Protruding-mouth mandibular arch skeleton chondrocyte ISH
Day 4 mandibular arch skeleton chondrocyte ISH
scfd1nju201Gt/nju201Gt standard conditions Protruding-mouth mandibular arch skeleton chondrocyte ISH
Day 4 mandibular arch skeleton chondrocyte ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Protruding-mouth chondrocyte extracellular space
IHC scfd1nju201Gt/nju201Gt standard conditions Protruding-mouth chondrocyte vesicle
Phenotype Details
Fish Conditions Stage Phenotype
scfd1nju201Gt/nju201Gt standard conditions Protruding-mouth chondrocyte vesicle ab1-col2a labeling mislocalised, abnormal
Day 4 mandibular arch skeleton chondrocyte col2a1a expression decreased amount, abnormal
scfd1nju201Gt/nju201Gt heat shock Protruding-mouth chondrocyte vacuolated, abnormal
Protruding-mouth chondrocyte endoplasmic reticulum membrane distended, abnormal
Protruding-mouth chondrocyte extracellular matrix morphology, abnormal
Protruding-mouth columnar chondrocyte disorganized, abnormal
Protruding-mouth retina cell disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(1), Hou, N., Yang, Y., Scott, I.C., Lou, X., The Sec domain protein Scfd1 facilitates trafficking of ECM components during chondrogenesis, 8-15, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.