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ZFIN ID: ZDB-FIG-170123-17
Miranda-Rodríguez et al., 2017 - RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos. Developmental Biology   421(1):27-42 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: 4-cell
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: 4-cell

Fig. 9

Inhibition of RhoA/ROCK pathway affects remodeling of the furrow microtubule array. Buffer (A, C and E) and C3, Rhosin and h1152 (B, D and F respectively) injected embryos labeled with anti α tubulin antibody. In buffer injected embryos the furrow microtubule array form as a parallel array at the center of the cleavage furrow at mature furrow (A, a1; C, c1 and E, e1) meanwhile in injected embryos with C3 (B, b1), Rhosin (D, d1) or h1152 (F, f1) no deposition of the microtubules at the center of the cleavage furrow was observed. Upon furrow maturation furrow microtubules appear at the distal edge in a characteristic V shape in buffer injected embryos (A, a2; C, c2 and E, e2). Microtubules from injected embryos with C3, Rhosin or h1152 did not showed this characteristic pattern (B, b2; D, d2 and F, f2 respectively) but maintained a perpendicular arrangement towards the cleavage furrow (b2 and d2) or showed a complete lack of organization (f2). Data was analyzed from 3 independent experiments of C3 injected embryos (n=11) or buffer injected embryos as control (n=7); from 4 independent experiments of Rhosin injected embryos (n=12) or buffer injected embryos as control (n=15) and from 4 independent experiments of h1152 injected embryos (n=13) or buffer injected as control (n=11).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-tuba IHC WT control 4-cell blastodisc cleavage furrow
IHC 4-cell blastodisc microtubule
IHC WT chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride 4-cell blastodisc cleavage furrow
IHC 4-cell blastodisc microtubule
IHC WT chemical treatment by injection: rhosin 4-cell blastodisc cleavage furrow
IHC 4-cell blastodisc microtubule
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by injection: (S)-2-methyl-1-(4-methylisoquinoline-5-sulfonyl)-1,4-diazepane dihydrochloride 4-cell blastodisc cleavage furrow structure, abnormal
4-cell blastodisc microtubule disorganized, abnormal
4-cell blastodisc mitotic cleavage furrow formation process quality, abnormal
WT chemical treatment by injection: rhosin 4-cell blastodisc cleavage furrow structure, abnormal
4-cell blastodisc microtubule disorganized, abnormal
4-cell blastodisc mitotic cleavage furrow formation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.