ZFIN ID: ZDB-FIG-170117-15
Expression analysis reveals that gsp/myoVb is expressed in the intestine of zebrafish larvae.
In situ hybridisation analyses on 5-day-old larvae using sense control probe (A, C) and anti-sense probe against myosin Vb (B, D) in whole mount (A, B) and post-staining isolated intestines (C, D) reveal that myosin Vb is expressed in the intestine. The histological sections of 4dpf (E, F) larvae stained using sense control probe (E) and anti-sense probe (F) show the presence of myosin Vb transcripts in the gut epithelium. RT-PCR analyses performed on cDNA from intestines isolated from 4 and 5 day-old larvae show that myosin Vb (G) and other two myosin V paralogues, Vaa and Vc (H) are expressed in the intestine. Abbreviation: M1 - 100 bp ladder marker. M2 - 1Kb ladder marker. The white arrows (in B and D) point towards the expression of myoVb. The black arrowhead in C points to the median fin-fold. The black arrows in (E, F) indicate the gut epithelium. r1 and r2 are PCR reactions 1 and 2, respectively. Note that the difference between the myo Vb amplicon size in G and H is due to two different primer pairs used. Scale bar in F is equal to 20 μm in E, F.
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Reprinted from Mechanisms of Development, 142, Sidhaye, J., Pinto, C.S., Dharap, S., Jacob, T., Bhargava, S., Sonawane, M., The zebrafish goosepimples/myosin Vb mutant exhibits cellular attributes of human microvillus inclusion disease, 62-74, Copyright
(2016) with permission from Elsevier.
Full text @ Mech. Dev.