header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-170113-5
Jiang et al., 2016 - MicroRNA-184 promotes differentiation of the retinal pigment epithelium by targeting the AKT2/mTOR signaling pathway. Oncotarget   7(32):52340-52353 Full text @ Oncotarget
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 4

Dre-miR-184 promotes retinal development in vivo. A-B. Relative expression of dre-miR-184 in zebrafish injected with dre-miR-184 inhibitor compared to NC inhibitor (A), and in embryos injected with dre-miR-184 mimic compared with NC mimic (B). C-D. Relative mRNA expressions of pax6a, rpe65c, rlbp1b, lrat, mertka, best1, krt18, cdh2, tjp1b, and ctnnb1 in zebrafish injected with dre-miR-184 inhibitor compared to NC inhibitor (C), and in embryos injected with dre-miR-184 mimic compared with NC mimic (D). *: p<0.05; **: p<0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
best1 TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
ctnnbip1 TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
krt18a.1 TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
lratb.2 TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
mertka TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
pax6a TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
rlbp1b TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
rpe65c TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
tjp1b TU control Day 4 whole organism RTPCR
TU + MO1-mir184 standard conditions Day 4 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-rpe65 IHC TU control Day 4 retinal pigmented epithelium
IHC TU + MO1-mir184 standard conditions Day 4 retinal pigmented epithelium
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-mir184 standard conditions Day 4 retinal pigment epithelium development disrupted, abnormal
Day 4 retinal pigmented epithelium Ab1-rpe65 labeling decreased amount, abnormal
Day 4 whole organism krt18a.1 expression decreased amount, abnormal
Day 4 whole organism rlbp1b expression decreased amount, abnormal
Day 4 whole organism tjp1b expression decreased amount, abnormal
Day 4 whole organism ctnnbip1 expression decreased amount, abnormal
Day 4 whole organism mertka expression decreased amount, abnormal
Day 4 whole organism lratb.2 expression decreased amount, abnormal
Day 4 whole organism rpe65c expression decreased amount, abnormal
Day 4 whole organism pax6a expression decreased amount, abnormal
Day 4 whole organism best1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget