FIGURE

Fig. 5

ID
ZDB-FIG-161230-9
Publication
Han et al., 2016 - Identification of novel genes and networks governing hematopoietic stem cell development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Identification of genes required for HSPC specification and validation in zebrafish

A. GO analysis of biological processes among Group IV target genes, showing enrichment of networks in the endothelial–hematopoietic transition.

B. Heat map showing bona fide HSC regulators in Group IV target genes.

C. Heat map showing the most depleted shRNAs in pairwise comparisons between the D20LSK and D20LK populations.

D. Validation of the involvement of Group X target genes Lrsam1, Ap2a1, Mettl22, and Hal in zebrafish hematopoiesis by MO‐mediated knockdown. Whole‐mount in situ hybridization of Runx1 and cMyb expression is shown at 26 hpf and 30 hpf, respectively. Prdm16 knockdown serves as a positive control. Note that nonspecific staining for Runx1 and c‐Myb was largely unaffected by targeted MO knockdown, whereas HSC markers Runx1 and c‐Myb staining were notably reduced in discrete cells in the ventral region of the dorsal aorta (arrows). Scale bar = 100 μm.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Rep.