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ZFIN ID: ZDB-FIG-161229-17
Venero Galanternik M. et al., 2016 - Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously. Developmental Biology   419(2):321-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

Bmp inhibition rescues Engrailed(+) muscle cells and cxcl12a expression in glypican4fr6. (A-C′) In situ hybridization shows expression of the Chemokine ligand cxcl12a in siblings (A-A′), glypican4fr6 No-turning mutants (B-B′) and glypican4fr6 U-turning mutants (C-C′) along the embryo trunk. (A′-C′) Higher magnification images of (A-C). (d-F) Engrailed (4D9) immunostaining reveals that, compared to siblings (D), glypican4fr6 No-turning mutants (E) and glypican4fr6 U-turning mutants (F) have a considerably diminished number of MPCs and MFFs. (G) Quantification of 4D9-positive muscle pioneer cells per somite at 24 hpf (p-values (t-test) for Control vs. No-turn=0.0036, Control vs. U-turn=5.09721×10−12, No-turn vs. U-turn=0.00065). Error bars represent standard error. (H-K) In control (H-I) and glypican4fr6 mutant (J-K) embryos inhibition of Bmp signaling with 10 μM Dorsomorphin induces the formation of MPCs and MFFs along the myoseptum. (L-O′) Bmp inhibition results in the upregulation of cxcl12a expression along the myoseptum in siblings (L-M′) and restores cxcl12a expression in glypican4fr6 mutants (N-O′) along the embryo trunk. (L′-O′) Higher magnification images of (L-O). (P-T) Inhibition of Bmp signaling does not affect primordium migration (P-Q) but significantly rescues the migration of glypican4fr6 mutants (R-S). (T) Quantification of primordia that have completed migration after Bmp inhibition, Fisher Exact Probability Test, Sibling-DMSO vs. Siblings-Dorsomorphin =0.31; glypican4fr6-DMSO vs. glypican4fr6-Dorsomorphin=0.04. Hours post treatment (hpt).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcl12a WT control Long-pec muscle pioneer ISH
WT chemical treatment by environment: dorsomorphin Long-pec muscle pioneer ISH
gpc4fr6/fr6 control Long-pec muscle pioneer ISH
gpc4fr6/fr6 chemical treatment by environment: dorsomorphin Long-pec muscle pioneer ISH
cxcr4b WT standard conditions Prim-5 horizontal myoseptum ISH
gpc4fr6/fr6 standard conditions Prim-5 horizontal myoseptum ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC WT control Long-pec muscle pioneer
IHC WT standard conditions Prim-5 muscle pioneer
IHC WT chemical treatment by environment: dorsomorphin Long-pec muscle pioneer
IHC gpc4fr6/fr6 control Long-pec muscle pioneer
IHC gpc4fr6/fr6 standard conditions Prim-5 muscle pioneer
IHC gpc4fr6/fr6 chemical treatment by environment: dorsomorphin Long-pec muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: dorsomorphin Long-pec muscle pioneer cxcl12a expression increased amount, abnormal
Long-pec muscle pioneer ab1-eng labeling increased amount, abnormal
gpc4fr6/fr6 standard conditions Prim-5 horizontal myoseptum cxcr4b expression decreased amount, abnormal
Prim-5 muscle pioneer ab1-eng labeling decreased amount, abnormal
gpc4fr6/fr6 chemical treatment by environment: dorsomorphin Long-pec muscle pioneer cxcl12a expression increased amount, abnormal
Long-pec muscle pioneer ab1-eng labeling increased amount, abnormal
Long-pec posterior lateral line neuromast primordium migration process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 419(2), Venero Galanternik M., Lush, M.E., Piotrowski, T., Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously, 321-335, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.